Tryptic y++ Fragment Ion Distributions Are Guided by Coulombic Repulsion

Ideal tryptic peptides contain only a single basic residue, located at the C-terminus. Collisional fragmentation of their doubly- or triply-protonated ions generates doubly-charged y ++ fragment ions with modest intensities. The size distribution of the y ++ fragments, when averaged over many spectr...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2012-03, Vol.23 (3), p.483-488
Main Authors: Irikura, Karl K., Merle, John K., Simón-Manso, Yamil
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bioinformatics
Biotechnology
Chemistry
Chemistry and Materials Science
Mass Spectrometry
Models, Molecular
Organic Chemistry
Peptide Fragments - chemistry
Proteomics
Protons
Research Article
Static Electricity
Trypsin - chemistry
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Summary:Ideal tryptic peptides contain only a single basic residue, located at the C-terminus. Collisional fragmentation of their doubly- or triply-protonated ions generates doubly-charged y ++ fragment ions with modest intensities. The size distribution of the y ++ fragments, when averaged over many spectra, corresponds closely to the expectations from charge-directed backbone cleavage and a Coulomb-Boltzmann distribution of mobile protons. This observation should be helpful in developing mechanistic models for y ++ formation.
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-011-0293-7