Efficacy of Passive Immunization with IgY Antibodies to a 58-kDa H. pylori Antigen on Severe Gastritis in BALB/c Mouse Model

• Published in: Journal of immunoassay & immunochemistry 2009-10, Vol.30 (4), p.359-377
• Main Authors: Attallah, Abdelfattah M., Abbas, Ayman T., Ismail, Hisham, Abdel-Raouf, Mohamed, El-Dosoky, Ibrahim
• Format: Article
• Language: English
• Subjects: 58-kDa antigen; Administration, Oral; Animal models; Animals; Antibodies; Antibodies, Bacterial - administration & dosage; Antibodies, Bacterial - immunology; Antibodies, Bacterial - therapeutic use; Antigens, Bacterial - immunology; BALB/c mice; Chickens - immunology; Disease Models, Animal; Female; Gastritis; Gastritis - microbiology; Gastritis - pathology; Gastritis - prevention & control; H. pylori; Helicobacter Infections - pathology; Helicobacter Infections - prevention & control; Helicobacter pylori; Helicobacter pylori - immunology; Humans; IgY antibodies; Immunization, Passive; Immunoglobulins - immunology; Immunoglobulins - therapeutic use; Mice; Mice, Inbred BALB C; Passive immunization
• ISSN: 1532-1819; 1532-4230
• DOI: 10.1080/15321810903187922

Consecutive triple doses of 1 × 10 8  CFU/mL of a pathogenic H. pylori strain isolated from stomach of Egyptian patients with severe gastritis were used to establish infection in BALB/c mice model. White Leghorn hens were immunized with H. pylori whole cell lysate (HpLysate) antigen and with a highly reactive 58-kDa H. pylori (Hp58) antigen. Two months later, IgY antibodies (IgY-HpLysate & IgY-Hp58) were purified from egg yolk and its efficacy was evaluated in the adopted model. Microbiological culture and immunohistochemical staining revealed that H. pylori infection was inhibited 1 week after oral passive immunization in 70% of infected BALB/c mice with a significant decrease (p