Basal and Stimulated Calcitonin and Procalcitonin by Various Assays in Patients with and without Medullary Thyroid Cancer

• Published in: Clinical chemistry (Baltimore, Md.) Md.), 2011-03, Vol.57 (3), p.467-474
• Main Authors: KRATZSCH, Jurgen, PETZOLD, Anne, REINERS, Christoph, THIERY, Joachim, DRALLE, Henning, FUHRER, Dagmar, RAUE, Friedhelm, REINHARDT, Walter, BRÖCKER-PREUSS, Martina, GÖRGES, Rainer, MANN, Klaus, KARGES, Wolfram, MORGENTHALER, Nils, LUSTER, Markus
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Automation; Bacterial diseases; Bacterial infections; Biological and medical sciences; Biomarkers, Tumor - blood; Calcitonin - blood; Calcitonin Gene-Related Peptide; Calibration; Carcinoma, Medullary - blood; Carcinoma, Medullary - diagnosis; Case-Control Studies; Cohort Studies; Female; Fundamental and applied biological sciences. Psychology; Humans; Immunoassay - methods; Investigative techniques, diagnostic techniques (general aspects); Luminescent Measurements - methods; Male; Medical sciences; Methods; Molecular biophysics; Pentagastrin; Protein Precursors - blood; Protein Stability; Reference Standards; Sensitivity and Specificity; Sex Characteristics; Studies; Thyroid; Thyroid cancer; Thyroid Neoplasms - blood; Thyroid Neoplasms - diagnosis; Thyroidectomy
• ISSN: 0009-9147; 1530-8561
• DOI: 10.1373/clinchem.2010.151688

Calcitonin (CT) is a sensitive marker for evaluation of medullary thyroid cancer (MTC). However, CT measurement can vary with assay- and nonassay-dependent factors, and procalcitonin (PCT) measurement has been proposed for evaluating questionable increases in CT. We tested 2 fully automated CT assays (Immulite [IL] and Liaison [LIA]) and 1 nonautomated CT assay (IRMA, Medipan) and compared these results with PCT (Brahms Kryptor). We evaluated preanalytical conditions and PCT cross-reactivity in sera of 437 patients with clinical conditions associated with hypercalcitoninemia. Additionally, we determined the true "nil" CT concentration in 60 thyroidectomized patients and defined CT cutoff concentrations for pentagastrin stimulation testing in 13 chronic kidney disease (CKD) patients and 10 MTC patients. Markedly decreased CT concentrations were found after storage of sera for >2 h at room temperature and >6 h at 4 °C. Cutoff concentrations for basal and stimulated CT were disease and assay dependent. Proton pump inhibitor therapy was the most frequent reason for increased CT. PCT concentrations were higher in patients with MTC than in patients with CKD without infections (P