Proliferation of rat molar pulp cells after direct pulp capping with dentine adhesive and calcium hydroxide

• Published in: Clinical oral investigations 2011-08, Vol.15 (4), p.577-587
• Main Authors: Dammaschke, Till, Stratmann, Udo, Fischer, Rudolf-Josef, Sagheri, Darius, Schäfer, Edgar
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Bacterial Infections - diagnosis; Bromodeoxyuridine; Calcium hydroxide; Calcium Hydroxide - therapeutic use; Cell adhesion; Cell Proliferation; Composite Resins - chemistry; Dental caries; Dental Leakage - classification; Dental Materials - chemistry; Dental pulp; Dental Pulp - cytology; Dental Pulp - drug effects; Dental Pulp - microbiology; Dental Pulp Capping - methods; Dental Pulp Exposure - drug therapy; Dental Pulp Necrosis - diagnosis; Dental Restoration, Permanent; Dentin-Bonding Agents - therapeutic use; Dentistry; DNA; Endothelial cells; Endothelial Cells - pathology; Female; Fibroblasts; Fibroblasts - pathology; Foreign bodies; Granulation Tissue - pathology; Indicators and Reagents; Infection; Inflammation; Male; Medicine; Mitotic Index; Molar - cytology; Molar - drug effects; Molars; Necrosis; Original Article; Pulp Capping and Pulpectomy Agents - therapeutic use; Rats; Rats, Wistar; Teeth; Time Factors
• ISSN: 1432-6981; 1436-3771
• DOI: 10.1007/s00784-010-0409-7

The aim was to evaluate the proliferation of pulp cells 1, 3 and 7 days after direct pulp capping with the dentine adhesive Gluma Comfort Bond (GCB) and to compare it with calcium hydroxide (Ca(OH) 2 ). An occlusal cavity was prepared in 72 molar teeth of 36 Wistar rats. Then GCB or Ca(OH) 2 was placed on the exposed pulp. All cavities were restored with composite. After 1, 3 and 7 days, the animals were sacrificed. One hour prior sacrification, 5-bromo-2′-desoxyuridine (BrdU) was injected into the intraperitoneal cavity for immunohistological analysis of 18 animals. BrdU was incorporated into the DNA to tag proliferating cells using an antibody staining. Three animals served as controls and were not further treated. The number of the tagged cells was statistically analysed by comparing the results of the three groups. In 18 rats, routine histological analysis was performed in order to evaluate the pulp tissue for bacterial infection, inflammatory cells and necrosis. The marked cells were identified as fibroblasts, endothelial cells (after 1, 3 and 7 days) and Höhl cells (after 7 days). One day after capping, significantly more cells were stained in the GCB than in the Ca(OH) 2 group ( p