Efficacy of Self-assembled Hydrogels Composed of Positively or Negatively Charged Peptides as Scaffolds for Cell Culture

KASEA16(+) and KASEA16(−) peptides, the net charges of which are positive and negative, respectively, under a neutral condition could undergo self-assembly into nanofibers and form transparent hydrogels without peptide aggregation upon rapid pH neutralization. The numbers of NIH3T3 cells attached to...

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Bibliographic Details
Published in:Journal of biomaterials applications 2012-02, Vol.26 (6), p.651-665
Main Authors: Nagayasu, Aya, Yokoi, Hidenori, Minaguchi, Jun A., Hosaka, Yoshinao Z., Ueda, Hiromi, Takehana, Kazushige
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biomedical materials
Cell Adhesion
Cell Proliferation
Charging
Culture
Effectiveness
Hydrogels
Hydrogels - chemistry
Mice
Molecular Sequence Data
Nanofibers - chemistry
Nanofibers - ultrastructure
NIH 3T3 Cells
Peptides
Peptides - chemistry
Scaffolds
Self assembly
Tissue Scaffolds - chemistry
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Summary:KASEA16(+) and KASEA16(−) peptides, the net charges of which are positive and negative, respectively, under a neutral condition could undergo self-assembly into nanofibers and form transparent hydrogels without peptide aggregation upon rapid pH neutralization. The numbers of NIH3T3 cells attached to the KASEA16(+) hydrogel and KASEA16(−) hydrogel were similar, and cells proliferated with time on both hydrogels. Cells on the KASEA16(+) hydrogel had spindle-like morphology, while cells on the KASEA16(−) hydrogel formed clusters without extending cytoplasmic processes. Comparison of differently charged peptides under a neutral condition suggested that the charges of the scaffolds should be taken into consideration for the best design and selection of scaffolds for cell culture. Since the KASEA16(+) peptide could form a stable hydrogel under a neutral condition and the hydrogel served as a scaffold for cell proliferation, the KASEA16(+) hydrogel will be a useful scaffold for cell culture.
ISSN:0885-3282
1530-8022
DOI:10.1177/0885328210379927