Hydrogenase activity monitoring in the fermentative hydrogen production using heat pretreated sludge: A useful approach to evaluate bacterial communities performance

The conversion of organic compounds into H 2 has received increasing attention. Enrichment of inocula by heat pretreatment eliminates non-spore forming hydrogen consuming microorganisms and promotes spore germination in genus Clostridium, which is known as one of the key hydrogen producers. Useful i...

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Bibliographic Details
Published in:International journal of hydrogen energy 2011-07, Vol.36 (13), p.7543-7549
Main Authors: de Sá, Lívian Ribeiro Vasconcelos, de Oliveira, Tatiane Corrêa, dos Santos, Thiago Ferreira, Matos, Andrea, Cammarota, Magali Christe, Oliveira, Edna Maria Morais, Ferreira-Leitão, Viridiana Santana
Format: Article
Language:English
Subjects:
Acetic acid
Alternative fuels. Production and utilization
Applied sciences
Bacteria
Biohydrogen
Energy
Exact sciences and technology
Fuels
Heat pretreatment
Hydrogen
Hydrogenase gene
Mixed culture
Real-time RT-PCR
Volatile fatty acids
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Summary:The conversion of organic compounds into H 2 has received increasing attention. Enrichment of inocula by heat pretreatment eliminates non-spore forming hydrogen consuming microorganisms and promotes spore germination in genus Clostridium, which is known as one of the key hydrogen producers. Useful information about metabolic pathway is provided by some intermediate metabolites, such as: acetic, propionic, butyric and formic acids. The increase of acetic/butyric acids ratio indicates H 2 production in heat pretreated inoculum when compared to untreated inoculum in the same cultivation conditions. The effect of heat pretreatment on inocula and consequently on the performance of bacterial communities responsible for H 2 production was monitored through the measurement of the level of hydrogenase gene expression, as well as through the content and distribution of volatile fatty acids. The acetic acid type fermentation was followed by the microorganisms presented in untreated and heat pretreated sludge. The medium containing untreated sludge presented a ratio of acetic/butyric acid of approximately 4, the same parameter was 7 when heat pretreated sludge was employed. The level of hydrogenase gene expression tripled when heat pretreated inoculum was used, indicating a higher production of H 2.
ISSN:0360-3199
1879-3487
DOI:10.1016/j.ijhydene.2011.03.119