Biomechanical and Histological Evaluations of the Doubled Semitendinosus Tendon Autograft After Anterior Cruciate Ligament Reconstruction in Sheep

• Published in: The American journal of sports medicine 2012-02, Vol.40 (2), p.315-324
• Main Authors: Kondo, Eiji, Yasuda, Kazunori, Katsura, Taro, Hayashi, Riku, Kotani, Yoshihisa, Tohyama, Harukazu
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; Anterior Cruciate Ligament - surgery; Anterior Cruciate Ligament Reconstruction; Biological and medical sciences; Biomechanical Phenomena; Biomechanics; Biomechanics. Biorheology; Diseases of the osteoarticular system; Female; Fundamental and applied biological sciences. Psychology; Knee; Knee Joint - surgery; Ligaments; Medical sciences; Models, Animal; Necrosis; Pneumonia; Sheep; Tendons; Tendons - pathology; Tendons - physiopathology; Tendons - transplantation; Tensile Strength; Tissues, organs and organisms biophysics; Wound Healing
• ISSN: 0363-5465; 1552-3365
• DOI: 10.1177/0363546511426417

Background: A sheep or goat anterior cruciate ligament (ACL) reconstruction model with the semitendinosus tendon autograft has not as of yet been established. Hypothesis: The semitendinosus tendon graft may be necrotized after ACL reconstruction until 12 weeks. Structural properties of the femur-graft-tibia complex may not be restored to the normal ACL level even at 12 months after surgery. Study Design: Controlled laboratory study. Methods: Thirty-eight mature sheep were used. In each animal, the right knee underwent ACL reconstruction using the semitendinosus tendon autograft. Two of them were not tested because of pneumonia. Twenty of 36 sheep were used for biomechanical evaluations. Five randomly selected animals were sacrificed at 0, 12, 24, or 52 weeks after surgery. In each animal, both the knees were harvested, and the left knee was used to obtain the control data. The remaining 16 sheep were used for histological evaluation. Two or 3 randomly selected animals were sacrificed at 0, 2, 6, 12, 24, or 52 weeks after surgery. Results: The tendon graft was predominantly acellular at 2 weeks. Although cell infiltration increased at 6 weeks, the core portion remained necrotic even at 12 weeks. At 24 and 52 weeks, the necrotic lesion disappeared in the core portion. In each period, the anteroposterior translation of the reconstructed knee remained significantly greater than that of the control (P < .0001). At 52 weeks, the maximum load of the femur-graft-tibia complex was significantly lower than that of the femur-ACL-tibia complex (P < .0001), although there was no significant difference in the cell density, the tissue dimensions, the stiffness, and the elongation at failure between the 2 complexes. Conclusion: The sheep ACL reconstruction model showed predominant intrinsic fibroblast necrosis in the tendon autograft until 12 weeks. Although the structural properties of the femur-graft-tibia complex gradually improved, they were not completely restored to the femur-ACL-tibia complex level even at 52 weeks. Clinical Relevance: Remodeling of the semitendinosus tendon autograft after ACL reconstruction is not different from that of the bone-tendon-bone graft. This study has suggested that vigorous activity should not be permitted for patients in the early periods after ACL reconstruction using semitendinosus tendon autografts, which are necrotized and weakened after surgery.