Selectable Gene Auto-Excision via a Cold Inducible "Gene Deletor" System

Selectable marker genes are necessary tools for selecting transformants during transgenic plant production. However, once transformation is accomplished, the presence of these selectable marker genes is no longer necessary and can even be undesirable. Here we describe the successful excision of sele...

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Bibliographic Details
Published in:African journal of agricultural research 2010-09, Vol.5 (17), p.2426-2433
Main Authors: Liu, Yang, Zhu, Youyin, Zhao, Degang, Lu, Litang
Format: Article
Language:English
Subjects:
Agronomy
Flowers & plants
Genetics
Science and Technology
Tobacco
Online Access:Get full text
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Summary:Selectable marker genes are necessary tools for selecting transformants during transgenic plant production. However, once transformation is accomplished, the presence of these selectable marker genes is no longer necessary and can even be undesirable. Here we describe the successful excision of selectable genes from transgenic plants via the use of a cold inducible 'gene deletor' system. During a transformation procedure in tobacco, transgenic plants obtained by selection on kanamycin medium and identified by GUS staining and PCR method. Results are reported.
ISSN:1991-637X
1991-637X