Highly sensitive spectrofluorimetic determination of rutin based on its activated effect on a multi-enzyme redox system

ABSTRACT A highly sensitive and simple spectrofluorimetric method for the determination of rutin, based on its activated effect on a haemoglobin‐catalysed reaction, was developed. Under optimum conditions, the concentration of rutin was linear, with decreased fluorescence (ΔF) of the system under op...

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Bibliographic Details
Published in:Luminescence (Chichester, England) England), 2012-01, Vol.27 (1), p.59-62
Main Authors: Chen, Ya-Hong, Tian, Feng-Shou
Format: Article
Language:English
Subjects:
activated
Calibration
Hemoglobins - chemistry
Limit of Detection
multi-enzyme redox system
Oxidation-Reduction
rutin
Rutin - analysis
Spectrometry, Fluorescence - methods
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Summary:ABSTRACT A highly sensitive and simple spectrofluorimetric method for the determination of rutin, based on its activated effect on a haemoglobin‐catalysed reaction, was developed. Under optimum conditions, the concentration of rutin was linear, with decreased fluorescence (ΔF) of the system under optimal experimental conditions. The calibration graph was linear in the range 1.0 × 10–7–3.0 × 10–5 mol/L, with a detection limit of 7.0 × 10–8 mol/L. The relative standard deviation (RSD) was 3.26% for 11 determinations of 1.0 × 10–5 mol/L. This method was used for the determination of rutin in pharmaceuticals with satisfactory results. Copyright © 2011 John Wiley & Sons, Ltd.
ISSN:1522-7235
1522-7243
DOI:10.1002/bio.1325