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Expression of the RNA recognition motif protein RBP10 promotes a bloodstream-form transcript pattern in Trypanosoma brucei
Summary When Trypanosoma brucei differentiates from the bloodstream form to the procyclic form, there are decreases in the levels of many mRNAs encoding proteins required for the glycolytic pathway, and the mRNA encoding the RNA recognition motif protein RBP10 decreases in parallel. We show that RBP...
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Published in: | Molecular microbiology 2012-03, Vol.83 (5), p.1048-1063 |
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creator | Wurst, Martin Seliger, Beate Jha, Bhaskar Anand Klein, Cornelia Queiroz, Rafael Clayton, Christine |
description | Summary
When Trypanosoma brucei differentiates from the bloodstream form to the procyclic form, there are decreases in the levels of many mRNAs encoding proteins required for the glycolytic pathway, and the mRNA encoding the RNA recognition motif protein RBP10 decreases in parallel. We show that RBP10 is a cytoplasmic protein that is specific to bloodstream‐form trypanosomes, where it is essential. Depletion of RBP10 caused decreases in many bloodstream‐form‐specific mRNAs, with increases in mRNAs associated with the early stages of differentiation. The changes were similar to, but more extensive than, those caused by glucose deprivation. Conversely, forced RBP10 expression in procyclics induced a switch towards bloodstream‐form mRNA expression patterns, with concomitant growth inhibition. Forced expression of RBP10 prevented differentiation of bloodstream forms in response to cis‐aconitate, but did not prevent expression of key differentiation markers in response to glucose deprivation. RBP10 was not associated with heavy polysomes, showed no detectable in vivo binding to RNA, and was not stably associated with other proteins. Tethering of RBP10 to a reporter mRNA inhibited translation, and halved the abundance of the bound mRNA. We suggest that RBP10 may prevent the expression of regulatory proteins that are specific to the procyclic form. |
doi_str_mv | 10.1111/j.1365-2958.2012.07988.x |
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When Trypanosoma brucei differentiates from the bloodstream form to the procyclic form, there are decreases in the levels of many mRNAs encoding proteins required for the glycolytic pathway, and the mRNA encoding the RNA recognition motif protein RBP10 decreases in parallel. We show that RBP10 is a cytoplasmic protein that is specific to bloodstream‐form trypanosomes, where it is essential. Depletion of RBP10 caused decreases in many bloodstream‐form‐specific mRNAs, with increases in mRNAs associated with the early stages of differentiation. The changes were similar to, but more extensive than, those caused by glucose deprivation. Conversely, forced RBP10 expression in procyclics induced a switch towards bloodstream‐form mRNA expression patterns, with concomitant growth inhibition. Forced expression of RBP10 prevented differentiation of bloodstream forms in response to cis‐aconitate, but did not prevent expression of key differentiation markers in response to glucose deprivation. RBP10 was not associated with heavy polysomes, showed no detectable in vivo binding to RNA, and was not stably associated with other proteins. Tethering of RBP10 to a reporter mRNA inhibited translation, and halved the abundance of the bound mRNA. We suggest that RBP10 may prevent the expression of regulatory proteins that are specific to the procyclic form.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.2012.07988.x</identifier><identifier>PMID: 22296558</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Binding sites ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Microbiology ; Oligonucleotide Array Sequence Analysis ; Parasitic protozoa ; Protein Biosynthesis ; Proteins ; Protozoan Proteins - genetics ; Protozoan Proteins - metabolism ; Ribonucleic acid ; RNA ; RNA Interference ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA, Protozoan - genetics ; RNA, Protozoan - metabolism ; Transcriptome ; Trypanosoma brucei ; Trypanosoma brucei brucei - genetics ; Trypanosoma brucei brucei - growth & development ; Trypanosoma brucei brucei - metabolism</subject><ispartof>Molecular microbiology, 2012-03, Vol.83 (5), p.1048-1063</ispartof><rights>2012 Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2012 Blackwell Publishing Ltd.</rights><rights>Copyright Blackwell Publishing Ltd. Mar 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5468-4e9af2b7191ca8bba238d3cd0676b936dc3c82f88b89973bd4ef636bc51f617e3</citedby><cites>FETCH-LOGICAL-c5468-4e9af2b7191ca8bba238d3cd0676b936dc3c82f88b89973bd4ef636bc51f617e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25589310$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22296558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wurst, Martin</creatorcontrib><creatorcontrib>Seliger, Beate</creatorcontrib><creatorcontrib>Jha, Bhaskar Anand</creatorcontrib><creatorcontrib>Klein, Cornelia</creatorcontrib><creatorcontrib>Queiroz, Rafael</creatorcontrib><creatorcontrib>Clayton, Christine</creatorcontrib><title>Expression of the RNA recognition motif protein RBP10 promotes a bloodstream-form transcript pattern in Trypanosoma brucei</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
When Trypanosoma brucei differentiates from the bloodstream form to the procyclic form, there are decreases in the levels of many mRNAs encoding proteins required for the glycolytic pathway, and the mRNA encoding the RNA recognition motif protein RBP10 decreases in parallel. We show that RBP10 is a cytoplasmic protein that is specific to bloodstream‐form trypanosomes, where it is essential. Depletion of RBP10 caused decreases in many bloodstream‐form‐specific mRNAs, with increases in mRNAs associated with the early stages of differentiation. The changes were similar to, but more extensive than, those caused by glucose deprivation. Conversely, forced RBP10 expression in procyclics induced a switch towards bloodstream‐form mRNA expression patterns, with concomitant growth inhibition. Forced expression of RBP10 prevented differentiation of bloodstream forms in response to cis‐aconitate, but did not prevent expression of key differentiation markers in response to glucose deprivation. RBP10 was not associated with heavy polysomes, showed no detectable in vivo binding to RNA, and was not stably associated with other proteins. Tethering of RBP10 to a reporter mRNA inhibited translation, and halved the abundance of the bound mRNA. We suggest that RBP10 may prevent the expression of regulatory proteins that are specific to the procyclic form.</description><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Microbiology</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Parasitic protozoa</subject><subject>Protein Biosynthesis</subject><subject>Proteins</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - metabolism</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Protozoan - genetics</subject><subject>RNA, Protozoan - metabolism</subject><subject>Transcriptome</subject><subject>Trypanosoma brucei</subject><subject>Trypanosoma brucei brucei - genetics</subject><subject>Trypanosoma brucei brucei - growth & development</subject><subject>Trypanosoma brucei brucei - metabolism</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkV9vFCEUxYnR2HX1KxhiYvRlRv4sDDz40Dbt2qSt2lTrG2EYRllnhhHYuOunl3HXNfHByAtw-Z2Tyz0AQIxKnNerVYkpZwWRTJQEYVKiSgpRbu6B2eHhPpghyVBBBfl0BB7FuEIIU8TpQ3BECJGcMTEDP842Y7AxOj9A38L0xcKb62MYrPGfB5emcu-Ta-EYfLJugDcn7zCabrlsI9Sw7rxvYgpW90XrQw9T0EM0wY0JjjolGwaYdbdhO-rBR99nSVgb6x6DB63uon2y3-fgw_nZ7emb4vLt8uL0-LIwbMFFsbBSt6SusMRGi7rWhIqGmgbxiteS8sZQI0grRC2krGjdLGzLKa8Nwy3HlaVz8GLnm5v-trYxqd5FY7tOD9avo5KEskogJjP58p8kRkQgyjKf0Wd_oSu_DkP-R_bLoyUsD3sOxA4ywccYbKvG4HodttlJTUGqlZryUlNeagpS_QpSbbL06d5_Xfe2OQh_J5eB53tAR6O7Ng_duPiHy4ykGGXu9Y777jq7_e8G1NXVxXTK-mKndzHZzUGvw1fFK1oxdXe9VOR8efKeiTv1kf4EZ_7JKQ</recordid><startdate>201203</startdate><enddate>201203</enddate><creator>Wurst, Martin</creator><creator>Seliger, Beate</creator><creator>Jha, Bhaskar Anand</creator><creator>Klein, Cornelia</creator><creator>Queiroz, Rafael</creator><creator>Clayton, Christine</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201203</creationdate><title>Expression of the RNA recognition motif protein RBP10 promotes a bloodstream-form transcript pattern in Trypanosoma brucei</title><author>Wurst, Martin ; Seliger, Beate ; Jha, Bhaskar Anand ; Klein, Cornelia ; Queiroz, Rafael ; Clayton, Christine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5468-4e9af2b7191ca8bba238d3cd0676b936dc3c82f88b89973bd4ef636bc51f617e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Binding sites</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Microbiology</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Parasitic protozoa</topic><topic>Protein Biosynthesis</topic><topic>Proteins</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - metabolism</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Protozoan - genetics</topic><topic>RNA, Protozoan - metabolism</topic><topic>Transcriptome</topic><topic>Trypanosoma brucei</topic><topic>Trypanosoma brucei brucei - genetics</topic><topic>Trypanosoma brucei brucei - growth & development</topic><topic>Trypanosoma brucei brucei - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wurst, Martin</creatorcontrib><creatorcontrib>Seliger, Beate</creatorcontrib><creatorcontrib>Jha, Bhaskar Anand</creatorcontrib><creatorcontrib>Klein, Cornelia</creatorcontrib><creatorcontrib>Queiroz, Rafael</creatorcontrib><creatorcontrib>Clayton, Christine</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wurst, Martin</au><au>Seliger, Beate</au><au>Jha, Bhaskar Anand</au><au>Klein, Cornelia</au><au>Queiroz, Rafael</au><au>Clayton, Christine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the RNA recognition motif protein RBP10 promotes a bloodstream-form transcript pattern in Trypanosoma brucei</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2012-03</date><risdate>2012</risdate><volume>83</volume><issue>5</issue><spage>1048</spage><epage>1063</epage><pages>1048-1063</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
When Trypanosoma brucei differentiates from the bloodstream form to the procyclic form, there are decreases in the levels of many mRNAs encoding proteins required for the glycolytic pathway, and the mRNA encoding the RNA recognition motif protein RBP10 decreases in parallel. We show that RBP10 is a cytoplasmic protein that is specific to bloodstream‐form trypanosomes, where it is essential. Depletion of RBP10 caused decreases in many bloodstream‐form‐specific mRNAs, with increases in mRNAs associated with the early stages of differentiation. The changes were similar to, but more extensive than, those caused by glucose deprivation. Conversely, forced RBP10 expression in procyclics induced a switch towards bloodstream‐form mRNA expression patterns, with concomitant growth inhibition. Forced expression of RBP10 prevented differentiation of bloodstream forms in response to cis‐aconitate, but did not prevent expression of key differentiation markers in response to glucose deprivation. RBP10 was not associated with heavy polysomes, showed no detectable in vivo binding to RNA, and was not stably associated with other proteins. Tethering of RBP10 to a reporter mRNA inhibited translation, and halved the abundance of the bound mRNA. We suggest that RBP10 may prevent the expression of regulatory proteins that are specific to the procyclic form.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22296558</pmid><doi>10.1111/j.1365-2958.2012.07988.x</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Binding sites Biological and medical sciences Fundamental and applied biological sciences. Psychology Gene expression Microbiology Oligonucleotide Array Sequence Analysis Parasitic protozoa Protein Biosynthesis Proteins Protozoan Proteins - genetics Protozoan Proteins - metabolism Ribonucleic acid RNA RNA Interference RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Protozoan - genetics RNA, Protozoan - metabolism Transcriptome Trypanosoma brucei Trypanosoma brucei brucei - genetics Trypanosoma brucei brucei - growth & development Trypanosoma brucei brucei - metabolism |
title | Expression of the RNA recognition motif protein RBP10 promotes a bloodstream-form transcript pattern in Trypanosoma brucei |
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