Changes in hormonal profile, gonads and sperm quality of Argyrosomus regius (Pisces, Scianidae) during the first sexual differentiation and maturation

• Published in: Theriogenology 2012-03, Vol.77 (5), p.888-898
• Main Authors: Schiavone, R, Zilli, L, Storelli, C, Vilella, S
• Format: Article
• Language: English
• Subjects: Anguilla anguilla; Animals; Argyrosomus regius; CASA; estradiol; Estradiol - blood; farmers; Female; females; Gonadal histology; gonadosomatic index; gonads; Gonads - growth & development; histology; Hormones - blood; Male; males; marine fish; Meagre; Perciformes - blood; Perciformes - growth & development; puberty; Sex Differentiation; Sex steroids; Sexual Maturation; spawning; Sperm Motility; spermatozoa; Spermatozoa - cytology; Spermatozoa - physiology; steroid hormones; surface area; testosterone; Testosterone - analogs & derivatives; Testosterone - blood
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2011.09.014

In the present study, sexual gonadal differentiation and first sexual maturation of Meagre (Argyrosomus regius) was studied, based upon the annual changes in gonadosomatic index (GSI), gonadal histology, and the plasma steroid hormones, testosterone (T), 11-ketotestosterone (11-KT), and estradiol (E2). In addition, spermatozoa characteristics were evaluated by measuring sperm motility and morphology. Results demonstrated that Meagre completes sex differentiation at 10 to 12 mo of age, and are group-synchronous spawners, which reach puberty at 2 (mean length 26.8 ± 0.7 cm, mean weight 920 ± 75 g; N = 10) and 3 (mean length 35.8 ± 0.8 cm, mean weight 1610 ± 89 g; N = 10) years of age for males and females, respectively. In males, during the sex differentiation period, T levels were significantly higher with respect to those of 11-KT; this suggests that T has a key role in the early phases of the sex differentiation. During the spawning season an increase in plasma concentrations of all hormones was observed with 11-KT levels being significantly higher that those of T. In females, during the sex differentiation period, there was an increase in E2 plasma levels, while during the first spawning season, a significant increase of T and E2 levels were measured. Regarding sperm characteristics, the measured curvilinear velocity (VCL) and straight-linear velocity (VSL), resulted in the same order of magnitude with respect to those measured in other marine fish, while the average path velocity (VAP) was similar to that measured in the European Eel. The head of Meagre spermatozoa presents as oval shaped with a surface area of approximately 3.66 μm² and a perimeter of approximately 6.65 μm. All these findings represent an important basis for further investigation on the reproductive biology of this specie and may assist the farmers to improve seed production in aquaculture.