Regulation of the differentiation of mesenchymal stem cells in vitro and osteogenesis in vivo by microenvironmental modification of titanium alloy surfaces

Abstract To mimic the extracellular microenvironment of bone, a bioactive multilayered structure of gelatin/chitosan pair, containing bone morphogenetic protein 2(BMP2) and fibronectin (FN), was constructed onto Ti6Al4V surface via a layer-by-layer assembly technique. The successful fabrication of m...

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Bibliographic Details
Published in:Biomaterials 2012-05, Vol.33 (13), p.3515-3528
Main Authors: Hu, Yan, Cai, Kaiyong, Luo, Zhong, Zhang, Yuan, Li, Liqi, Lai, Min, Hou, Yanhua, Huang, Yuran, Li, Jinghua, Ding, Xingwei, Zhang, Bin, Paul Sung, K.L
Format: Article
Language:English
Subjects:
Advanced Basic Science
Alkaline Phosphatase - metabolism
Animals
BMP2
Bone and Bones - diagnostic imaging
Bone and Bones - radiation effects
Bone Morphogenetic Protein 2 - pharmacology
Cell Adhesion - drug effects
Cell Differentiation - drug effects
Cell Shape - drug effects
Cellular Microenvironment - drug effects
Coated Materials, Biocompatible - pharmacology
Dentistry
Extracellular microenvironment
Gene Expression Regulation - drug effects
Humans
Implants, Experimental
Layer-by-layer assembly technique
Mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - enzymology
Mice
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Osteoclasts - cytology
Osteoclasts - drug effects
Osteoclasts - metabolism
Osteogenesis
Osteogenesis - drug effects
Rabbits
Rats
RNA, Messenger - genetics
RNA, Messenger - metabolism
Surface Properties - drug effects
Ti6Al4V alloy
Titanium - pharmacology
Water - chemistry
X-Ray Microtomography
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Summary:Abstract To mimic the extracellular microenvironment of bone, a bioactive multilayered structure of gelatin/chitosan pair, containing bone morphogenetic protein 2(BMP2) and fibronectin (FN), was constructed onto Ti6Al4V surface via a layer-by-layer assembly technique. The successful fabrication of multilayered structure was confirmed by contact angle measurement, field emission scanning electron microscopy (FE-SEM) and confocal laser scanning microscopy (CLSM), respectively. Bioactive BMP2 released in a sustained manner along with the degradation of multilayered structure. MSCs grown onto the multilayer coated TC4 substrates displayed significantly higher ( p  
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2012.01.040