Structural Variation of the Superintegron in the Toxigenic Vibrio cholerae O1 El Tor

• Published in: Biomedical and environmental sciences 2011-12, Vol.24 (6), p.579-592
• Main Authors: GAO, Yan, PANG, Bo, WANG, Hai Yin, ZHOU, Hai Jian, CUI, Zhi Gang, KAN, Biao
• Format: Article
• Language: English
• Subjects: Cassette; Cholera - epidemiology; Cholera - microbiology; Chromosomes, Bacterial - genetics; Chromosomes, Bacterial - ultrastructure; Cluster Analysis; DNA, Bacterial - genetics; Electrophoresis, Gel, Pulsed-Field; Gene Deletion; Gene Flow; Genetic Variation; Humans; Integrons - genetics; Mutagenesis, Insertional; Open Reading Frames - genetics; Polymerase Chain Reaction; SIS; Superintegron; Tandem Repeat Sequences; Vibrio cholerae; Vibrio cholerae O1 - classification; Vibrio cholerae O1 - genetics; Vibrio cholerae O1 - isolation & purification; Vibrio cholerae O1 - ultrastructure; 产毒; 厄尔尼诺; 拟态弧菌; 聚合酶链反应; 遗传结构; 重复序列; 霍乱弧菌
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/0895-3988.2011.06.001

Objective To understand the genetic structures and variations of the superintegron （Sl） in Vibrio cholerae isolated in the seventh cholera pandemic. Methods Polymerase chain reaction scanning and fragment sequencing were used. Sixty toxigenic V. cholerae O1 El Tor strains isolated between 1961 and 2008 were analyzed. Results Some variations were found, including insertions, replacements, and deletions. Most of the deletions were probably the result of recombination between V. cholerae repeat sequences. The majority of the variations clustered together. The Sis of the strains isolated in the 1960s and 1970s showed more diversity, whereas SI cassette variations in strains isolated in the 1990s and after were lower, with -24 kb signature sequence deletion. This indicates the predominant Sl in the host during the epidemic in the 1990s and after. The insertion cassettes suggested the mobilization from the Sls of other V. cholerae serogroups and Vibrio mimicus. Conclusion The study revealed that structural variations of Sis were obvious in the strains isolated in epidemics in different decades, whereas the divergence was based on syntenic structure of Sis in these El Tot strains. Also, the continuing cassette flows in the Sis of the host strains during the seventh cholera pandemics were displayed.