Fluorescence dye as novel label molecule for quantitative SERS investigations of an antibiotic

Within this contribution, the proof-of-principle for a new concept for indirect surface-enhanced Raman spectroscopy (SERS) detection is presented. The fluorescence dye FR-530 is applied as a label molecule for the antibiotic erythromycin. The antibiotic binds directly to the label molecule. Changes...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2012-03, Vol.402 (8), p.2625-2631
Main Authors: März, Anne, Trupp, Sabine, Rösch, Petra, Mohr, Gerhard J., Popp, Jürgen
Format: Article
Language:English
Subjects:
Analytical Chemistry
Anti-Bacterial Agents - analysis
Biochemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Erythromycin
Erythromycin - analysis
Fluorescence
Fluorescent Dyes - chemistry
Food Science
Investigations
Laboratory Medicine
Molecular Structure
Monitoring/Environmental Analysis
Original Paper
Raman spectroscopy
Spectrum Analysis, Raman
Surface Properties
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Summary:Within this contribution, the proof-of-principle for a new concept for indirect surface-enhanced Raman spectroscopy (SERS) detection is presented. The fluorescence dye FR-530 is applied as a label molecule for the antibiotic erythromycin. The antibiotic binds directly to the label molecule. Changes within the SERS spectrum of the fluorescence dye appearing with the presence of the antibiotic are utilized for the detection and quantitative investigations of erythromycin. With the new concept of binding the label molecule directly to the analyte molecule, the application of linkage compounds like antibodies or any other recognition molecules becomes dispensable.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-011-5273-z