Survivin siRNA Inhibits Gastric Cancer in Nude Mice

The objective of the study was to evaluate the expression of survivin, cell proliferation, and apoptosis in survivin-specific siRNA-transfected human gastric cancer cell line MGC-803. For this purpose, the target gene fragments were cloned into pSilencer3.1-Hl neo vector. Recombinant eukaryotic expr...

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Bibliographic Details
Published in:Cell biochemistry and biophysics 2012-03, Vol.62 (2), p.337-341
Main Authors: Wenying, Zhao, Zhaoning, Ji, Zhimin, Yang, Dongyun, Chen, Lili, Sheng
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biotechnology
Cell Biology
Cell Line, Tumor
Cell Proliferation - drug effects
G2 Phase Cell Cycle Checkpoints
Gene Expression Regulation, Neoplastic - drug effects
Humans
Inhibitor of Apoptosis Proteins - genetics
Inhibitor of Apoptosis Proteins - metabolism
Life Sciences
M Phase Cell Cycle Checkpoints
Mice
Mice, Nude
Original Paper
Pharmacology/Toxicology
Repressor Proteins - genetics
Repressor Proteins - metabolism
RNA Interference
RNA, Small Interfering - metabolism
RNA, Small Interfering - pharmacology
Stomach Neoplasms - pathology
Transplantation, Heterologous
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Summary:The objective of the study was to evaluate the expression of survivin, cell proliferation, and apoptosis in survivin-specific siRNA-transfected human gastric cancer cell line MGC-803. For this purpose, the target gene fragments were cloned into pSilencer3.1-Hl neo vector. Recombinant eukaryotic expression vector, pSilencer3.1-SVV was successfully constructed and then the recombinant vector was transfected into gastric cancer MGC-803 cells. The mRNA expression of survivin was determined by reverse-transcriptase polymerase chain reaction (RT-PCR). Survivin protein expression was detected by Western blot. Cell cycle distribution and apoptosis were determined by flow cytometry. Our data regarding RT-PCR and Western blot showed that pSilencer3.1-SVV vector could knockdown the expression of survivin mRNA and protein. In contrast with the control group, the apoptotic index of MGC-803 cells increased remarkably. Survivin-specific siRNA caused cells accumulation in the G2/M phase and the number of cells in the G0/G1 phase decreased after transfection. It was, therefore, concluded that the siRNA targeting survivin gene could inhibit the proliferation of gastric cancer cells and induce apoptosis. The use of survivin siRNA may provide a novel approach for gene therapy of gastric cancer.
ISSN:1085-9195
1559-0283
DOI:10.1007/s12013-011-9315-0