Resveratrol prevents streptozotocin-induced diabetes by inhibiting the apoptosis of pancreatic β-cell and the cleavage of poly (ADP-ribose) polymerase

• Published in: Endocrine Journal 2012, Vol.59(2), pp.103-109
• Main Authors: Ku, Cheol Ryong, Lee, Hyeon Jeong, Kim, Suk Kyoung, Lee, Eun Young, Lee, Mi-Kyung, Lee, Eun Jig
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - therapeutic use; Apoptosis - drug effects; Blood Glucose - analysis; Caspase 3 - metabolism; Diabetes Mellitus, Type 1 - blood; Diabetes Mellitus, Type 1 - metabolism; Diabetes Mellitus, Type 1 - pathology; Diabetes Mellitus, Type 1 - prevention & control; Enzyme Activation - drug effects; Glucose Tolerance Test; Hyperglycemia - prevention & control; Hypoglycemic Agents - therapeutic use; In Situ Nick-End Labeling; Insulin-Secreting Cells - drug effects; Insulin-Secreting Cells - metabolism; Insulin-Secreting Cells - pathology; Male; Pancreatic β-cell; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) polymerase; Poly(ADP-ribose) Polymerases - metabolism; Proteolysis - drug effects; Rats; Rats, Sprague-Dawley; Resveratrol; Stilbenes - therapeutic use; Streptozocin; Streptozotocin; Time Factors
• ISSN: 0918-8959; 1348-4540
• DOI: 10.1507/endocrj.EJ11-0194

Resveratrol (3,5,4’-trihydroxystilbene; RSV) is one kind of polyphenolic phytoalexin that has many effects on metabolic diseases. This study aimed to evaluate the protective effect of RSV pretreatment on β-cell. Male Sprague Dawley rats weighing 200-230 g were divided into 4 groups: (1) RSV; (2) streptozotocin (STZ, 70 mg/kg, intraperitoneally); (3) STZ after 7 days pretreatment with RSV; and (4) STZ pretreated with nicotinamide. Fasting glucose concentration was measured and an intraperitoneal glucose tolerance test was performed 72 h after STZ injection to determine the diabetic condition. The pancreas was removed 3, 6, 36, and 48 h after STZ injection. STZ induced diabetes in all rats not given RSV pretreatment, whereas none of the RSV-pretreated rats developed diabetes. Pretreatment with RSV inhibited apoptosis and reduced the activation of caspase-3 and poly(ADP-ribose) polymerase (PARP). However, expression of the total length PARP was not affected by pretreatment. Our findings suggest that RSV protects β-cells from STZ simultaneously with inhibiting the activation of PARP.