Comparison of two commercial enzyme-linked immunosorbent assays for the diagnosis of Porcine reproductive and respiratory syndrome virus infection

• Published in: Journal of veterinary diagnostic investigation 2012-03, Vol.24 (2), p.344-348
• Main Authors: Díaz, Ivan, Venteo, Ángel, Rebollo, Belén, Martín-Valls, Gerard E., Simon-Grifé, Meritxell, Sanz, Antonio, Mateu, Enric
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Viral - blood; Enzyme-Linked Immunosorbent Assay - veterinary; Nucleocapsid Proteins - chemistry; Nucleocapsid Proteins - immunology; Porcine Reproductive and Respiratory Syndrome - blood; Porcine Reproductive and Respiratory Syndrome - diagnosis; Porcine Reproductive and Respiratory Syndrome - virology; Porcine respiratory and reproductive syndrome virus; Porcine respiratory and reproductive syndrome virus - isolation & purification; Sensitivity and Specificity; Swine
• ISSN: 1040-6387; 1943-4936
• DOI: 10.1177/1040638711435804

Early diagnosis of Porcine reproductive and respiratory syndrome virus (PRRSV) is critically important for control of the disease. Two new commercially available enzyme-linked immunosorbent assays (ELISAs) based on different methodologies have been developed. In the present report, the 2 ELISAs were compared using blood samples from experimentally and naturally infected pigs. One of the 2 ELISAs was shown to be more sensitive than the other. The higher sensitivity of one of the ELISAs could pose a problem in PRRS diagnosis in endemic farms, because it can detect maternally derived antibodies for a longer time, overlapping with the detection of antibodies developed after PRRSV infection. However, the ELISA with higher sensitivity could be suitable for early detection of PRRSV antibodies in individual pigs, especially in PRRS-free herds.