Diabetes-impaired wound healing and altered macrophage activation: A possible pathophysiologic correlation

Macrophages play a critical role in wound healing and can be activated to two distinctive phenotypes in vitro: classical macrophage activation (caM) and alternative macrophage activation (aaM). This study investigated whether the impaired cutaneous repair observed in streptozotocin‐induced diabetic...

Full description

Saved in:
Bibliographic Details
Published in:Wound repair and regeneration 2012-03, Vol.20 (2), p.203-213
Main Authors: Miao, Mingyuan, Niu, Yiwen, Xie, Ting, Yuan, Bo, Qing, Chun, Lu, Shuliang
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Diabetes Mellitus, Experimental - immunology
Diabetes Mellitus, Experimental - physiopathology
Immunohistochemistry
Interleukin-10 - metabolism
Interleukin-12 - metabolism
Macrophage Activation
Male
Phenotype
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Skin - immunology
Skin - injuries
Skin - physiopathology
Wound Healing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Macrophages play a critical role in wound healing and can be activated to two distinctive phenotypes in vitro: classical macrophage activation (caM) and alternative macrophage activation (aaM). This study investigated whether the impaired cutaneous repair observed in streptozotocin‐induced diabetic rats was associated with altered macrophage activation. Our results show that macrophage activation phenotypes could be observed in wound healing through double immunostaining. The caM macrophages appeared in the initial stage of wound healing, followed by aaM macrophages, which predominated in normal wounds. However, through examining markers associated with activation by immunoblotting and real‐time polymerase chain reaction (PCR), diabetic wounds demonstrated insufficient caM in the early stage but excessive aaM in the later proliferative phase. Moreover, the macrophage activation markers were correlated with the instructive T helper cell type 1 (Th1)/Th2 cytokines in both groups. It was indicated that changed macrophage activation might contribute to impaired healing in diabetes wounds, and that strategies for reverting this abnormal activation could be useful for enhancing the wound healing process.
ISSN:1067-1927
1524-475X
DOI:10.1111/j.1524-475X.2012.00772.x