Numerical Analysis of Two-Phase Electrophoresis

Numerical modeling results for electrophoresis in aqueous two-phase systems are shown to agree with experimental results in both batch and flow devices. A previously discovered polarization effect due to the phase partitioning is further investigated. Numerical and experimental results indicate that...

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Bibliographic Details
Published in:Separation science and technology 2011-06, Vol.46 (10), p.1546-1554
Main Authors: Clark, William M., Lindblad, Mark A.
Format: Article
Language:English
Subjects:
aqueous two-phase
Devices
Electric field strength
Electrophoresis
flow device
liquid-liquid interface
Mathematical models
numerical modeling
Optimization
Partitioning
Polarization
Separation
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Summary:Numerical modeling results for electrophoresis in aqueous two-phase systems are shown to agree with experimental results in both batch and flow devices. A previously discovered polarization effect due to the phase partitioning is further investigated. Numerical and experimental results indicate that this polarization effect is not always significant since it is small for systems with equilibrium partition coefficients near 1 and since its influence diminishes with increasing electric field strength and electrophoresis time. The numerical models should prove useful for optimizing two-phase electrophoretic separations aimed at either concentrating solutes near the interface or directing them across it.
ISSN:0149-6395
1520-5754
DOI:10.1080/01496395.2011.573518