Development of Electrochemiluminescent Biosensor for Choline Based on Carbon Nanotubes Modified Electrode

An electrochemiluminescent (ECL) choline biosensor was developed by drop-coating of choline oxidase (ChOx) onto a carbon nanotubes (CNTs)/potassium ferricyanide modified platinum electrode with ECL of luminol as readout signal. Due to the improvement of biocompatibility and electron transfer of elec...

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Bibliographic Details
Published in:Fēnxī huàxué 2011-07, Vol.39 (7), p.985-989
Main Authors: Zhao, Jin-Jin, Wu, Mei-Sheng, Tu, Yi-Feng
Format: Article
Language:chi ; eng
Subjects:
Biosensors
Carbon nanotubes
Choline
Dispersions
Electrodes
Emission analysis
Enzymes
Platinum
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Summary:An electrochemiluminescent (ECL) choline biosensor was developed by drop-coating of choline oxidase (ChOx) onto a carbon nanotubes (CNTs)/potassium ferricyanide modified platinum electrode with ECL of luminol as readout signal. Due to the improvement of biocompatibility and electron transfer of electrode surface from CNTs, meanwhile the activation for enzyme and the ECL emission from K(3)Fe(CN)(6), the developed biosensor possesses excellent analytical properties. The biosensor gives optimal results while the Pt electrode was modified with 4 mu L of 0.33 g/L CNTs dispersoid, 2 mu L of 0.1 mol/L K(3)Fe(CN)(6) and 1.5 U of ChOx. In the PBS buffer (pH 7.4) containing 8x10(-6)mol/L luminol, the ECL signal linearly responded the concentration of choline from 1 x 10(-7) mol/L to 4x10(-3) mol/L 0=0.994) with detection limit of 1.21 x 10(-8) mol/L under 30 degree C of detection temperature. The developed biosensor was applied to assay the concentration of choline in rat blood sample. The result of 0.268 mg/100 mL was obtained with average recovery of 101.1%. It shows a fast response to choline with good reproducibility.
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2011.00985