A Novel and Highly Sensitive Resonance Scattering Spectral Assay for Horseradish Peroxidase Using Cationic Surfactant

A novel and ultrasensitive resonance scattering (RS) spectral assay was proposed for detection of horseradish peroxidase (HRP) activity. It was based on that the HRP strongly catalyze H 2 O 2 oxidation of excess I − to form , the resulting combined with four cationic surfactant (CS), including tetra...

Full description

Saved in:
Bibliographic Details
Published in:Journal of fluorescence 2008-11, Vol.18 (6), p.1059-1064
Main Authors: Liang, Ai-Hui, Jiang, Bo, Ma, Ji, Jiang, Zhi-Liang, Li, Ji-Shun
Format: Article
Language:English
Subjects:
Ammonium bromides
Ammonium chlorides
Analytical Chemistry
Assaying
Biochemistry
Biological and Medical Physics
Biological Assay
Biomedical and Life Sciences
Biomedicine
Biophysics
Biotechnology
Cationic
Cations - chemistry
Horseradish Peroxidase - metabolism
Hydrogen Peroxide - chemistry
Kinetics
Original Paper
Oxidation-Reduction
Peroxidase
Resonance scattering
Scattering, Radiation
Spectra
Spectrophotometry
Surface-Active Agents - chemistry
Surfactants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A novel and ultrasensitive resonance scattering (RS) spectral assay was proposed for detection of horseradish peroxidase (HRP) activity. It was based on that the HRP strongly catalyze H 2 O 2 oxidation of excess I − to form , the resulting combined with four cationic surfactant (CS), including tetradecyl pyridinium bromide (TPB), cetyltrimethyl ammonium bromide (CTMA), cetylpyridinium chloride (CPCM) and tetradecyl dimethylbenzyl ammonium chloride (TDMBA) to produce association particles (TPB-I 3 ) n , (CTMA-I 3 ) m , (CPCM-I 3 ) l and (TDMBA-I 3 ) k , which exhibit a strongest resonance scattering peak at 478, 423, 538 and 491 nm, respectively. For the four systems of TPB, CPCM, CTMBA and TDMBA, the HRP activity determined was in the linear range of 0.004–5.6, 0.04–3.2, 0.04–8.0, 0.08–8.0 ng/mL, with a detection limit of 0.0034, 0.040, 0.033, 0.016 ng/mL, respectively. The TPB resonance scattering spectral assay was best and has been applied to the analysis of HRP in real samples, with satisfactory results.
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-008-0350-1