Nanosecond electric pulses cause mitochondrial membrane permeabilization in Jurkat cells

Nanosecond, high‐voltage electric pulses (nsEP) induce permeabilization of the plasma membrane and the membranes of cell organelles, leading to various responses in cells including cytochrome c release from mitochondria and caspase activation associated with apoptosis. We report here evidence for ns...

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Bibliographic Details
Published in:Bioelectromagnetics 2012-04, Vol.33 (3), p.257-264
Main Authors: Napotnik, Tina Batista, Wu, Yu-Hsuan, Gundersen, Martin A., Miklavčič, Damijan, Vernier, P. Thomas
Format: Article
Language:English
Subjects:
Benzoxazoles
Cell Membrane Permeability
Electricity
Fluoresceins
Humans
Jurkat Cells
Membrane Potential, Mitochondrial - physiology
mitochondrial membrane potential
nanosecond electric pulse
Organometallic Compounds
permeabilization
plasma membrane
Quinolinium Compounds
Rhodamine 123
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Summary:Nanosecond, high‐voltage electric pulses (nsEP) induce permeabilization of the plasma membrane and the membranes of cell organelles, leading to various responses in cells including cytochrome c release from mitochondria and caspase activation associated with apoptosis. We report here evidence for nsEP‐induced permeabilization of mitochondrial membranes in living cells. Using three different methods with fluorescence indicators—rhodamine 123 (R123), tetramethyl rhodamine ethyl ester (TMRE), and cobalt‐quenched calcein—we have shown that multiple nsEP (five pulses or more, 4 ns duration, 10 MV/m, 1 kHz repetition rate) cause an increase of the inner mitochondrial membrane permeability and an associated loss of mitochondrial membrane potential. These effects could be a consequence of nsEP permeabilization of the inner mitochondrial membrane or the activation of mitochondrial membrane permeability transition pores. Plasma membrane permeabilization (YO‐PRO‐1 influx) was detected in addition to mitochondrial membrane permeabilization. Bioelectromagnetics 33:257–264, 2012. © 2011 Wiley Periodicals, Inc.
ISSN:0197-8462
1521-186X
DOI:10.1002/bem.20707