High evolutionary rate of human astrovirus

• Published in: Infection, genetics and evolution genetics and evolution, 2012-03, Vol.12 (2), p.435-442
• Main Authors: Babkin, Igor V., Tikunov, Artem Y., Zhirakovskaia, Elena V., Netesov, Sergei V., Tikunova, Nina V.
• Format: Article
• Language: English
• Subjects: Amino Acid Substitution; Astrovirus; children; elderly; etiological agents; evolution; Evolution, Molecular; gastroenteritis; Genetic Variation; genome; Human astrovirus; Humans; Mamastrovirus - classification; Mamastrovirus - genetics; Mutation Rate; Open Reading Frames; people; Phylogeny; Recombination; RNA; RNA, Viral; Virus evolution; viruses
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2012.01.019

► Full genome sequencing of four HAstV strains from Novosibirsk, Russia was performed. ► Eleven potential recombination breakpoints in HAstV genome were found. ► For the first time the rate of HAstV evolution was calculated. ► The rate of HAstV evolution is 3×10–3 nucleotide substitutions per site per year. Human astrovirus is one of the etiological agents of acute gastroenteritis in humans, mostly in young children and elderly people. Complete genome sequencing of four human astrovirus strains isolated in Novosibirsk, Russia was performed. Analysis of these sequences and the sequences available in GenBank database has detected numerous potential recombination breakpoints. For the first time the rate of human astrovirus evolution was estimated based on the genome fragments without recombination breakpoints; the determined rate is typical of the RNA viruses with high evolutionary rate, amounting to approximately 3.7×10–3 nucleotide substitutions per site per year, and for the synonymous changes, 2.8×10–3 nucleotide substitutions per site per year.