ZnO/Cu Nanocomposite: A Platform for Direct Electrochemistry of Enzymes and Biosensing Applications

• Published in: Langmuir 2012-03, Vol.28 (9), p.4580-4585
• Main Authors: Yang, Chi, Xu, Chunxiang, Wang, Xuemei
• Format: Article
• Language: English
• Subjects: Biosensing Techniques - methods; Chemistry; Copper - chemistry; Electrochemistry; Electrochemistry - methods; Exact sciences and technology; General and physical chemistry; Microscopy, Electron, Scanning; Nanocomposites - chemistry; Nanocomposites - ultrastructure; Zinc Oxide - chemistry
• ISSN: 0743-7463; 1520-5827
• DOI: 10.1021/la2044202

Unique structured nanomaterials can facilitate the direct electron transfer between redox proteins and the electrodes. Here, in situ directed growth on an electrode of a ZnO/Cu nanocomposite was prepared by a simple corrosion approach, which enables robust mechanical adhesion and electrical contact between the nanostructured ZnO and the electrodes. This is great help to realize the direct electron transfer between the electrode surface and the redox protein. SEM images demonstrate that the morphology of the ZnO/Cu nanocomposite has a large specific surface area, which is favorable to immobilize the biomolecules and construct biosensors. Using glucose oxidase (GOx) as a model, this ZnO/Cu nanocomposite is employed for immobilization of GOx and the construction of the glucose biosensor. Direct electron transfer of GOx is achieved at ZnO/Cu nanocomposite with a high heterogeneous electron transfer rate constant of 0.67 ± 0.06 s–1. Such ZnO/Cu nanocomposite provides a good matrix for direct electrochemistry of enzymes and mediator-free enzymatic biosensors.