NMR structural analysis of the capsular polysaccharide from Streptococcus pneumoniae serotype 6C

The original structure of Streptococcus pneumoniae capsular polysaccharide (CPS) serotype 6C was proposed based on chemical degradation and tandem mass analysis [J. Clin. Microbiol.2007, 45, 1225-1233]. In order to confirm the repeat unit structure and assign the stereochemical structure, the CPS 6C...

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Bibliographic Details
Published in:Carbohydrate research 2012-04, Vol.351, p.98-107
Main Authors: Cai, Ping, Moran, Justin, Pavliak, Viliam, Deng, Chenghua, Khoury, Nancy, Marcq, Olivier, Ruppen, Mark E
Format: Article
Language:English
Subjects:
Capsules
Carbohydrate Sequence
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Monosaccharides - chemistry
Polysaccharides, Bacterial - chemistry
Quality Control
Stereoisomerism
Streptococcus pneumoniae - chemistry
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Summary:The original structure of Streptococcus pneumoniae capsular polysaccharide (CPS) serotype 6C was proposed based on chemical degradation and tandem mass analysis [J. Clin. Microbiol.2007, 45, 1225-1233]. In order to confirm the repeat unit structure and assign the stereochemical structure, the CPS 6C and the known CPS 6A were fully characterized by NMR spectroscopy. Full (1)H and (13)C NMR spectra assignments of CPS 6C and CPS 6A were achieved based on DQCOSY, TOCSY, HSQC, HMBC, and NOESY analysis. These analyses confirmed the published structure of CPS 6A and established the repeat unit structure of the CPS 6C as: →2)-α-D-Glcp-(1→3)-α-D-Glcp-(1→3)-α-L-Rhap-(1→3)-D-Ribitol-(5→phosphate-.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2012.01.017