Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform

We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynam...

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Bibliographic Details
Published in:Lab on a chip 2012-04, Vol.12 (7), p.1289-1295
Main Authors: Burger, Robert, Reith, Patrick, Kijanka, Gregor, Akujobi, Victor, Abgrall, Patrick, Ducrée, Jens
Format: Article
Language:English
Subjects:
Antibodies - immunology
Arrays
Assaying
Beads
Centrifugation
Dimethylpolysiloxanes - chemistry
Humans
Immunoassay
Immunoglobulin G - chemistry
Immunoglobulin G - immunology
Instrumentation
Microfluidic Analytical Techniques - instrumentation
Microfluidics
Multiplexing
Particle Size
Platforms
Polystyrenes - chemistry
Sedimentation
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Summary:We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic conditions. The impact parameters governing the occupancy distribution and capture efficiency of the arrayed traps are investigated. The unique, nearly 100% capture efficiency paired with the capability to establish sharply peaked, single occupancy distributions enables a novel, digital readout mode for color-multiplexed, particle-based assays with low-complexity instrumentation. The presented technology marks an essential step towards a versatile platform for the integration of bead- and cell-based biological assays.
ISSN:1473-0197
1473-0189
DOI:10.1039/c2lc21170j