Biocontrol of Fusarium head blight: interactions between Trichoderma and mycotoxigenic Fusarium

Fusarium head blight (FHB) is a re-emerging wheat disease that causes extensive damage through direct losses in yield and quality due to the presence of damaged Fusarium kernels and their associated mycotoxins such as the trichothecene deoxynivalenol (DON). Biological control, including the treatmen...

Full description

Saved in:
Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2012-01, Vol.158 (Pt 1), p.98-106
Main Authors: Matarese, F, Sarrocco, S, Gruber, S, Seidl-Seiboth, V, Vannacci, G
Format: Article
Language:English
Subjects:
Antibiosis
Fusarium - genetics
Fusarium - metabolism
Fusarium culmorum
Molecular Sequence Data
Mycotoxins - metabolism
Oryza - microbiology
Oryza sativa
Pest Control, Biological
Plant Diseases - microbiology
Plant Diseases - prevention & control
Soil Microbiology
Trichoderma
Trichoderma - genetics
Trichoderma - isolation & purification
Trichoderma - physiology
Triticum - microbiology
Triticum aestivum
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fusarium head blight (FHB) is a re-emerging wheat disease that causes extensive damage through direct losses in yield and quality due to the presence of damaged Fusarium kernels and their associated mycotoxins such as the trichothecene deoxynivalenol (DON). Biological control, including the treatment of crop residues with antagonists, in order to reduce pathogen inoculum of FHB, holds considerable promise. Ten Trichoderma isolates, previously selected for their ability to grow in the presence of DON, were preliminarily investigated as potential antagonists against Fusarium culmorum and F. graminearum mycotoxigenic strains in plate confrontation assays. The three Trichoderma isolates showing antibiosis and mycoparasitism were evaluated for their capacity to inhibit DON production by F. graminearum and F. culmorum on two natural substrates. The expression of some chitinase-encoding genes by the two best resulting Trichoderma strains, during interaction with F. culmorum and F. graminearum, was monitored. All investigated genes from chitinase subgroups A, B and the new subgroup C responded to mycoparasitic conditions and were upregulated before contact and/or when in contact with the host. T. gamsii 6085, the best antagonist, was finally used in a competition test against F. culmorum and F. graminearum on natural substrates, using a qPCR approach to evaluate its effect on the pathogen's growth and DON production in haulms and rice. This test confirmed the ability of T. gamsii 6085 to antagonize the pathogens on rice. On wheat haulms, an extreme oligotrophic environment, T. gamsii 6085 seemed to develop very poorly and the growth of both the pathogens was unaffected by the presence of the antagonist.
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.052639-0