Natural occurrence of lethal aspergillosis in the cattle tick Rhipicephalus (Boophilus) microplus (Acari:Ixodidae)

• Published in: Parasitology 2012-02, Vol.139 (2), p.259-263
• Main Authors: MIRANDA-MIRANDA, E., COSSIO-BAYUGAR, R., MARTÍNEZ-IBAÑEZ, F., CASASANERO-ORDUÑA, R., FOLCH-MALLOL, J.
• Format: Article
• Language: English
• Subjects: Acari; Aflatoxins; Animals; Arachnida; Aspergillosis; Aspergillus flavus - isolation & purification; Biological and medical sciences; Cattle; Female; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Invertebrates; Rhipicephalus - microbiology; Rhipicephalus - ultrastructure
• ISSN: 0031-1820; 1469-8161; 1469-8161
• DOI: 10.1017/S0031182011001843

The purpose of this study was to describe an unreported entomopathogenic fungus that naturally infects the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Engorged female ticks, showed symptoms of fungal infection after controlled tick infestation of cattle. Infected ticks developed a distinctive dark colour, a pale mould grew over the cuticle and the ticks eventually died covered with fungal conidiophores. The responsible fungus was isolated and cultured on mycological medium and submitted to microscopic morphology, biochemical phenotyping and 18S rRNA ribotyping analyses, which identified it as aflatoxin-producing Aspergillus flavus. Spores from the cultured fungus were experimentally sprayed over healthy engorged female ticks, obtaining an 80% prevalence of experimental infection of healthy ticks and their egg masses, the larval progeny after incubation under laboratory conditions was also infected. These results demonstrate that A. flavus is the causative agent of the natural fungal disease of the cattle tick R. microplus described here.