Purification and inflammatory edema induced by two PLA sub(2) (Anch TX-I and Anch TX-II) from sea anemone Anthothoe chilensis (Actiniaria: Sagartiidae)

The Anch TX-I and II PLA sub(2) were purified from Anthothoe chilensis (Lesson, 1830) from the extract of the anemone after only two chromatographic step using molecular exclusion chromatography (Sephadex G-75) and reverse phase HPLC on mu -Bondapak C18 column. Both PLA sub(2) showed a molecular mas...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology Part B: Biochemistry and Molecular Biology, 2012-02, Vol.161 (2), p.170-177
Main Authors: Landucci, Elen Cristina Teizem, Dias, Queila Cristina, Marangoni, Fabio Andre, Vilca-Quispe, Augusto, Valeriano-Zapana, Jose Antonio, Torres-Huaco, Frank Denis, Martins-De-Souza, Daniel, Marangoni, Sergio, Ponce-Soto, Luis Alberto
Format: Article
Language:English
Subjects:
Actiniaria
Anthothoe chilensis
Bombus pennsylvanicus
Bombus terrestris
Bunodosoma caissarum
Cnidaria
Heloderma horridum
Heloderma suspectum
Lacertilia
Marine
Sagartiidae
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Summary:The Anch TX-I and II PLA sub(2) were purified from Anthothoe chilensis (Lesson, 1830) from the extract of the anemone after only two chromatographic step using molecular exclusion chromatography (Sephadex G-75) and reverse phase HPLC on mu -Bondapak C18 column. Both PLA sub(2) showed a molecular mass of ~ 14 kDa determined by MALDI-TOF mass spectrometry and showed a high catalytic activity (data not showed). Although homologous with mammalian or snake venom group I PLA sub(2)s, Anch TX-I and II is sufficiently structurally different for the question of its placement into the existing PLA sub(2) classification scheme to arise. In addition, Anch TX-I and II, despite possessing many common structural features, also differ in some important structural properties. The amino acid sequence of both PLA sub(2) (Anch TX-I and III) showed high amino acid sequence identity with PLA sub(2)Rhopilema nomadica and Bunodosoma caissarum Cnidaria and PLA sub(2) of group III protein isolated from the Mexican lizard Heloderma horridum horridum and Heloderma suspectum. In addition, Anch TX-I and Anch TX-II showed high amino acid sequence identity with PLA sub(2) from group III also showed significant overall homology to bee Apis dorsata, Bombus terrestris and Bombus pennsylvanicus and PLA sub(2). We also investigated the in vivo edematogenic activity of Anch TX-I and Anch TX-II in a model of paw and skin edema in rats and observed that both are able to induce dose-dependent edema.
ISSN:1096-4959
DOI:10.1016/j.cbpb.2011.11.003