A broad spectrum, one-step RT-PCR to detect Satsuma dwarf virus variants using universal primers targeting both segmented RNAs 1 and 2

Universal primers to detect Satsuma dwarf virus (SDV), including distantly related strains Citrus mosaic virus (CiMV), Navel orange infectious mottling virus (NIMV), and Hyuganatsu virus (HV), were tested in a convenient one-step RT-PCR assay. SDV was the most broadly detected using uSDVup/uSDVdo pr...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2011-11, Vol.77 (6), p.326-330
Main Authors: Shimizu, Shin-ichi, Ito, Takao, Miyoshi, Takanori, Tachibana, Yasunobu, Ito, Tsutae
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Citrus
Citrus fruits
Genetic diversity
Life Sciences
Microbiology
Plant diseases
Plant Pathology
Satsuma dwarf virus
Trees
Viral and Viroid Diseases
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Universal primers to detect Satsuma dwarf virus (SDV), including distantly related strains Citrus mosaic virus (CiMV), Navel orange infectious mottling virus (NIMV), and Hyuganatsu virus (HV), were tested in a convenient one-step RT-PCR assay. SDV was the most broadly detected using uSDVup/uSDVdo primers that specifically targeted a nucleotide sequence in the 3′-noncoding region that is conserved in both segmented RNAs 1 and 2 of SDV among the tested primers. Nucleotide sequence analysis confirmed that the amplified RT-PCR products could be derived from RNAs 1 or 2 of SDV variants, some of which had interesting genetic diversity.
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-011-0342-x