Construction of Streptomyces nogalater Lv65 strains with enhanced nogalamicin biosynthesis using regulatory genes

Influence of cloned regulatory genes on nogalamycin biosynthesis by Streptomyces nogalater LV65 strain has been studied. Gene snorA from the S. nogalater genome was cloned in multicopy replicative plasmid pSOKA and integrative plasmid pR3A. Introduction of these plasmids into S. nogalater wild type...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2011-11, Vol.47 (6), p.594-598
Main Authors: Klimishin, D. A, Rabyk, M. V, Gren’, T. P, Nimets’, O. Ya, Gonchar, M. A, Gromyko, A. N, Fedorenko, V. A
Format: Article
Language:English
Subjects:
antibiotics
Biochemistry
Biomedical and Life Sciences
Biosynthesis
chromosomes
Cloning
gene expression
Genes
Life Sciences
Medical Microbiology
Microbiology
nogalamycin
plasmids
regulator genes
Streptomyces
Streptomyces coelicolor
Streptomyces ghanaensis
Streptomyces globisporus
Streptomyces nogalater
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Summary:Influence of cloned regulatory genes on nogalamycin biosynthesis by Streptomyces nogalater LV65 strain has been studied. Gene snorA from the S. nogalater genome was cloned in multicopy replicative plasmid pSOKA and integrative plasmid pR3A. Introduction of these plasmids into S. nogalater wild type cells resulted in enhanced nogalamicin biosynthesis. A similar effect was observed at heterologous expression of gene (p)ppGpp-synthetase gene relA cloned from Streptomyces coelicolor A3(2). Heterologous expression of genes absA2 from Streptomyces ghanaensis ATCC14672 and lndYR from genome Streptomyces globisporus 1912 decreased synthesis of antibiotic. The study results indicate the presence of homologs of these genes in chromosome of S. nogalater, their possible participation in regulation of nogalamicin biosynthesis, and provide us with a possibility for genetic design of the strains with higher synthesis of this antibiotic.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683811060068