High-density genetic mapping for coffee leaf rust resistance

Coffee leaf rust caused by the fungus Hemileia vastatrix causes considerable economic losses for coffee producers. Although agrochemical products can provide sufficient disease control, the use of resistant cultivars is a safer alternative. This resistance may be constrained by one or a few genetic...

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Bibliographic Details
Published in:Tree genetics & genomes 2011-12, Vol.7 (6), p.1199-1208
Main Authors: Diola, Valdir, de Brito, Giovani Greigh, Caixeta, Eveline Teixeira, Maciel-Zambolim, Eunize, Sakiyama, Ney Sussumu, Loureiro, Marcelo Ehlers
Format: Article
Language:English
Subjects:
Agrochemicals
amplified fragment length polymorphism
Biomedical and Life Sciences
Biotechnology
chromosome mapping
Cloning
Coffee
crossing
Cultivars
Disease control
dominant genes
Forestry
fungi
Genes
Genetic engineering
Genetic factors
genetic markers
Genomes
Hemileia vastatrix
Hybrids
leaf rust
Leaves
Life Sciences
marker-assisted selection
Original Paper
Pathogens
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Polymorphism
Tree Biology
Trees
Virulence
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Summary:Coffee leaf rust caused by the fungus Hemileia vastatrix causes considerable economic losses for coffee producers. Although agrochemical products can provide sufficient disease control, the use of resistant cultivars is a safer alternative. This resistance may be constrained by one or a few genetic factors, mainly those found in material originating from interspecific hybrids. In this study, the genetic analysis of an F 2 population consisting of 224 plants derived from a crossing of Híbrido de Timor UFV 427-15 (resistant) with Catuaí Amarelo IAC 30 (susceptible) showed that a dominant gene confers the resistance of coffee to race II of H. vastatrix. From a genetic map saturated with 25 amplified fragment length polymorphism (AFLP) markers linked to the resistance gene, we developed a high-density genetic map with six sequence-characterized amplified region (SCAR) markers delimiting a chromosomal region of 9.45 cM and flanking the dominant gene at 0.7 and 0.9 cM. This is the first saturated and high-density genetic map obtained from this region containing the resistance gene. The results of this study are of great importance for the introduction of molecular markers for marker-assisted selection; they will also facilitate studies related to the cloning, structure, and function of race-specific genes involved in the resistance of coffee trees to H. vastatrix.
ISSN:1614-2942
1614-2950
DOI:10.1007/s11295-011-0406-2