Cd super(2+) binds to Atx1 and affects the physical interaction between Atx1 and Ccc2 in Saccharomyces cerevisiae

The ATX1 deletion strain of Saccharomyces cerevisiae is more resistant to Cd super(2+) than the wild-type. To investigate the function of Atx1 in Cd super(2+) toxicity, we used a metal-binding assay to study the interaction between Atx1 and Cd super(2+) in vitro. Using circular dichroism and two-hyb...

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Bibliographic Details
Published in:Biotechnology letters 2012-02, Vol.34 (2), p.303-307
Main Authors: Heo, Dong-Hyuk, Baek, In-Joon, Kang, Hyun-Jun, Kim, Ji-Hyun, Chang, Miwha, Kang, Chang-Min, Yun, Cheol-Won
Format: Article
Language:English
Subjects:
Binding
Biotechnology
Deletion
Dichroism
In vitro testing
Saccharomyces cerevisiae
Strain
Toxicity
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Summary:The ATX1 deletion strain of Saccharomyces cerevisiae is more resistant to Cd super(2+) than the wild-type. To investigate the function of Atx1 in Cd super(2+) toxicity, we used a metal-binding assay to study the interaction between Atx1 and Cd super(2+) in vitro. Using circular dichroism and two-hybrid analyses, we found that Atx1 can bind Cd super(2+) specifically and that Cd super(2+) binding to Atx1 affects the physical interaction between Atx1 and Ccc2. These results imply that Atx1 delivers Cd super(2+) to Ccc2 and that this delivery is, at least in part, responsible for Cd super(2+) toxicity in S. cerevisiae.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-011-0763-9