Infectious bursal disease DNA vaccination conferring protection by delayed appearance and rapid clearance of invading viruses

The present study was undertaken to determine the kinetics of viral load and immune response in protection against infectious bursal disease virus (IBDV) by DNA vaccination. Chickens were DNA-vaccinated and challenged with IBDV one week after the third vaccination. Tissues were collected at 12 hours...

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Bibliographic Details
Published in:Archives of virology 2011-12, Vol.156 (12), p.2241-2250
Main Authors: Chen, Yung-Yi, Hsieh, Ming Kun, Tung, Chun-Yu, Wu, Ching Ching, Lin, Tsang Long
Format: Article
Language:English
Subjects:
Amino acids
Animals
Antibodies
Antibodies, Neutralizing - blood
Antibodies, Viral - blood
Avian Proteins - genetics
Base Sequence
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Birnaviridae Infections - immunology
Birnaviridae Infections - prevention & control
Birnaviridae Infections - veterinary
Birnaviridae Infections - virology
Bursa of Fabricius - immunology
Bursa of Fabricius - virology
Chickens
Fundamental and applied biological sciences. Psychology
Infections
Infectious bursal disease virus
Infectious bursal disease virus - genetics
Infectious bursal disease virus - immunology
Infectious bursal disease virus - isolation & purification
Infectious Diseases
Interferon-gamma - genetics
Interleukin-4 - genetics
Medical Microbiology
Microbiology
Miscellaneous
Original Article
Peptides
Poultry
Poultry Diseases - immunology
Poultry Diseases - prevention & control
Poultry Diseases - virology
Proteins
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Viral - analysis
RNA, Viral - genetics
Spleen
Spleen - virology
T-Lymphocyte Subsets - immunology
Vaccines
Vaccines, DNA - pharmacology
Veterinary colleges
Veterinary medicine
Viral infections
Viral Vaccines - pharmacology
Virology
Virulence
Viruses
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Description
Summary:The present study was undertaken to determine the kinetics of viral load and immune response in protection against infectious bursal disease virus (IBDV) by DNA vaccination. Chickens were DNA-vaccinated and challenged with IBDV one week after the third vaccination. Tissues were collected at 12 hours postinfection (HPI), 1 day postinfection (DPI), 3, 5, 7 and 10 DPI. The vaccinated chickens had less viral RNA, with delayed appearance and shorter duration in the bursa of Fabricius, spleen, and cecal tonsil than the challenged control chickens. Their ELISA and neutralizing antibody titers were decreased at 12 HPI and significantly lower (P 
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-011-1127-4