Biotechnological conversion of glycerol to 2-amino-1,3-propanediol (serinol) in recombinant Escherichia coli

Microbial conversion is an important technology for the refinement of renewable resources. Here, we describe the biotechnological conversion of glycerol to 2-amino-1,3-propanediol (serinol), a relevant intermediate in several chemical syntheses processes. Either the dihydroxyacetone phosphate aminot...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2012, Vol.93 (1), p.357-365
Main Authors: Andreessen, Björn, Steinbüchel, Alexander
Format: Article
Language:English
Subjects:
Acinetobacter
Acinetobacter - enzymology
Acinetobacter - genetics
Applied Microbial and Cell Physiology
Batch culture
Biodiesel fuels
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Bradyrhizobium - enzymology
Bradyrhizobium - genetics
Carbon
Detoxification
Dihydroxyacetone Phosphate - metabolism
E coli
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - metabolism
Gene Expression
Glycerol
Glycerol - metabolism
Life Sciences
Metabolic Engineering
Microbial Genetics and Genomics
Microbiology
Organisms, Genetically Modified
Plasmids
Propanolamines
Propylene Glycols - metabolism
Renewable resources
RNA polymerase
Studies
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Summary:Microbial conversion is an important technology for the refinement of renewable resources. Here, we describe the biotechnological conversion of glycerol to 2-amino-1,3-propanediol (serinol), a relevant intermediate in several chemical syntheses processes. Either the dihydroxyacetone phosphate aminotransferase/dihydrorhizobitoxine synthase (RtxA) of Bradyrhizobium elkanii USD94 or only the N-terminal domain (RtxA513) comprising the first reaction, respectively, was expressed in recombinant Escherichia coli . Serinol contents of up to 3.3 g/l were achieved in batch cultures. We could further clarify that glutamic acid is the preferred cosubstrate for the transamination of dihydroxyacetone phosphate to serinolphosphate, which is the essential step in serinol synthesis. An in vivo detoxification of serinol employing wax ester synthase/acyl-CoA:diacyl-glycerol acyl transferase from Acinetobacter baylyi ADP1 was not accomplished. This study paves the way for biotechnological production of serinol from glycerol derived from the biodiesel industry.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-011-3364-6