Effect of melatonin on DNA damage of bovine cumulus cells during in vitro maturation (IVM) and on in vitro embryo development

The effect of melatonin during in vitro maturation (IVM) on DNA damage of cumulus cells (CCs) from bovine cumulus–oocyte complexes (COCs) and embryo development was evaluated. COCs from abattoir ovaries were cultured in maturation medium (MM) with 0.5 μg/ml FSH and 5.0 μg/ml LH (FSH–LH); 10 −9 M mel...

Full description

Saved in:
Bibliographic Details
Published in:Research in veterinary science 2012-02, Vol.92 (1), p.124-127
Main Authors: Takada, L., Junior, A. Martins, Mingoti, G.Z., Balieiro, J.C.C., Cipolla-Neto, J., Coelho, L.A.
Format: Article
Language:English
Subjects:
Animals
Antioxidants - administration & dosage
Antioxidants - pharmacology
Apoptosis
Aquaculture
Bovine
Cattle
Cells, Cultured
Comet assay
Comet Assay - veterinary
Comets
Culture Media
Cumulus cells
Cumulus Cells - physiology
DNA Damage - drug effects
Embryo Culture Techniques - veterinary
Embryonic Development - drug effects
Embryos
Experiments
Fertilization in Vitro - veterinary
Free radicals
Gonadotropins
In vitro fertilization
In Vitro Oocyte Maturation Techniques - methods
In Vitro Oocyte Maturation Techniques - veterinary
Melatonin
Melatonin - administration & dosage
Melatonin - pharmacology
Mineral oils
Studies
Veterinary medicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effect of melatonin during in vitro maturation (IVM) on DNA damage of cumulus cells (CCs) from bovine cumulus–oocyte complexes (COCs) and embryo development was evaluated. COCs from abattoir ovaries were cultured in maturation medium (MM) with 0.5 μg/ml FSH and 5.0 μg/ml LH (FSH–LH); 10 −9 M melatonin (MEL) or FSH–LH + MEL (FSH–LH–MEL). After 24 h of in vitro maturation, the CCs surrounding the oocyte were subjected to DNA analysis by Comet assay. After in vitro fertilization and in vitro embryo culture, the embryo development rates were evaluated on day 2 post insemination (cleavage) and days 7–8 (blastocyst). The percentage of CCs with no DNA damage was significantly superior in MEL group (37.6 ± 2.4) than in FSH–LH–MEL (28.0 ± 2.4) and FSH–LH (17.8 ± 2.41) groups. Cleavage and blastocysts rates were similar among groups. Melatonin during IVM protects the CCs from DNA damage but this effect did not influence embryo development in vitro.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2010.11.004