Oxidative Macromolecular Alterations in the Rat Central Nervous System in Response to Experimentally Co-Induced Chlorpyrifos and Cold Stress: A Comparative Assessment in Aging Rats

Reactive oxygen species are generated as a result of a number of physiological and pathological processes which can promote multiple forms of oxidative damage including protein oxidation, and thereby influence the function of a diverse array of cellular processes. In our previous study we have repor...

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Bibliographic Details
Published in:Neurochemical research 2012-02, Vol.37 (2), p.335-348
Main Authors: Basha, P. Mahaboob, Poojary, Annappa
Format: Article
Language:English
Subjects:
Aging
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Central Nervous System - drug effects
Central Nervous System - metabolism
Chlorpyrifos - pharmacology
Cholinesterase Inhibitors - pharmacology
Cold Temperature
Male
Neurochemistry
Neurology
Neurosciences
Original Paper
Oxidation-Reduction
Rats
Rats, Wistar
Stress, Physiological
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Summary:Reactive oxygen species are generated as a result of a number of physiological and pathological processes which can promote multiple forms of oxidative damage including protein oxidation, and thereby influence the function of a diverse array of cellular processes. In our previous study we have reported that co-exposure to chlorpyrifos and cold stress in aging rats markedly influence the toxic outcome as a result of oxidative stress. In the present study, key neurochemical/enzymes were measured in order to evaluate the macromolecular alterations in response to experimentally co-induced chlorpyrifos and cold stress (15 and 20°C) either concurrently or individually in vivo for 48 h in discrete regions of brain and spinal cord of different age group rats. CPF and cold stress exposure either individually or in combination substantially increased the activity/levels of protein carbonyls, AST, ALT and decreased protein thiols, DNA, RNA and total proteins in discrete regions of CNS. Overall, the effects of co-exposure were appreciably different from either of the exposures. However, synergistic-action of CPF and cold stress at 15°C showed higher dyshomeostasis in comparison with CPF and cold stress alone and together at 20°C indicating the extent of oxidative macromolecular damage in discrete regions of brain and spinal cord. Furthermore, the present study demonstrates that macromolecular oxidative damage is highly pronounced in neonates and juveniles than the young adults.
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-011-0617-9