A comparison of neurotoxicity in cerebellum produced by dermal application of chlorpyrifos in young and adult mice

Chlorpyrifos (CPF), an organophosphate pesticide inhibits acetylcholinesterase (AChE) and causes neuromuscular incoordination among children and elderly. The objectives of the present study were to compare the neurotoxic effects of dermal application of CPF on the cerebellum in the parameters of gli...

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Bibliographic Details
Published in:Journal of Neural Transmission 2012-03, Vol.119 (3), p.345-352
Main Authors: Krishnan, K., Mitra, N. K., Yee, L. S., Yang, H. M.
Format: Article
Language:English
Subjects:
Acetylcholinesterase
Acetylcholinesterase - blood
Administration, Cutaneous
Age Factors
Animals
Astrocytes - drug effects
Astrocytes - metabolism
Basic Neurosciences
Cerebellum
Cerebellum - drug effects
Cerebellum - metabolism
Children
Chlorpyrifos
Chlorpyrifos - administration & dosage
Chlorpyrifos - toxicity
Cholinesterase Inhibitors - administration & dosage
Cholinesterase Inhibitors - toxicity
Genetics and Immunology - Original Article
Geriatrics
Glial cells
Glial fibrillary acidic protein
Glial Fibrillary Acidic Protein - metabolism
Insecticides - administration & dosage
Insecticides - toxicity
Male
Medicine
Medicine & Public Health
Medulla
Mice
Mice, Inbred BALB C
Neurology
Neurosciences
Neurotoxicity
Paraffin
Pesticides (organophosphorus)
Psychiatry
Skin
Tails
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Summary:Chlorpyrifos (CPF), an organophosphate pesticide inhibits acetylcholinesterase (AChE) and causes neuromuscular incoordination among children and elderly. The objectives of the present study were to compare the neurotoxic effects of dermal application of CPF on the cerebellum in the parameters of glial fibrillary acidic protein (GFAP) expression in young and adult mice and to correlate with the changes in acetylcholinesterase levels. Male Balb/c mice, 150 days old (adult) and 18 days old (young) were dermally applied with ½ LD 50 of CPF over the tails for 14 days. Serum AChE concentration was estimated and GFAP immunostaining was performed on sagittal paraffin sections through the vermis of cerebellum. Although reduced in both age-groups exposed to CPF, percentage of reduction in serum AChE was more in adult compared to the young. Under GFAP immunostaining, brown colour fibres and glial cells were observed in cerebellar cortex and medulla in both the experimental groups. The mean GFAP-positive glial cell count in cerebellar medulla per mm 2 of section was significantly ( p  
ISSN:0300-9564
1435-1463
DOI:10.1007/s00702-011-0715-5