Metagenomic analysis of DNA viruses in a wastewater treatment plant in tropical climate

• Published in: Environmental microbiology 2012-02, Vol.14 (2), p.441-452
• Main Authors: Tamaki, Hideyuki, Zhang, Rui, Angly, Florent E., Nakamura, Shota, Hong, Pei-Ying, Yasunaga, Teruo, Kamagata, Yoichi, Liu, Wen-Tso
• Format: Article
• Language: English
• Subjects: Biomass; DNA Viruses - classification; DNA Viruses - genetics; Metagenome; Metagenomics; Sewage - virology; Tropical Climate; Waste Disposal, Fluid; Water Microbiology
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/j.1462-2920.2011.02630.x

Summary Viruses have been detected in the different stages of wastewater treatment plants (WWTPs) at concentrations of 108–1010 ml−1 of virus‐like particles (VLPs), 10–1000 times higher than in natural aquatic environments, suggesting that WWTPs can be considered as an important reservoir and source of viruses. This study revealed novel diversity and function with the DNA viral communities in the influent, activated sludge, anaerobic digester, and effluent of a domestic WWTP using metagenomics. WWTP was a very specific environment, with less than 5% of the > 936 000 metagenomic sequences obtained (∼70–119 Mbp per sample) similar to sequences present in other environmental viromes. Many viruses found in the WWTP were novel, resulting in only  82% were shared. These data suggested that VLPs of most viral types could be present between 1 and 30 days in the process before they were discharged. Viruses in WWTP and the discharged ones can have potential impacts on the functioning of the wastewater treatment system and on the dynamics of microbial community in the surrounding aquatic environments respectively.