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Anti-urolithiatic effects of Punica granatum in male rats
In Ayurvedic medicine, the Punica granatum is considered “a Pharmacy unto itself”. In the present study we found that the chloroform and methanol extract of Punica granatum fruits has a role in preventing Urolithiasis in male rats. The traditional use of Punica granatum has been reported to regulate...
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| Published in: | Journal of ethnopharmacology 2012-03, Vol.140 (2), p.234-238 |
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| container_title | Journal of ethnopharmacology |
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| creator | Rathod, N.R. Biswas, Dipak Chitme, H.R. Ratna, Sanjeev Muchandi, I.S. Chandra, Ramesh |
| description | In Ayurvedic medicine, the Punica granatum is considered “a Pharmacy unto itself”. In the present study we found that the chloroform and methanol extract of Punica granatum fruits has a role in preventing Urolithiasis in male rats.
The traditional use of Punica granatum has been reported to regulate urine discharge and controls the burning sensation of urine.
Animals model of calcium oxalate urolithiasis was developed in male rats by adding ethylene glycol 0.75% in drinking water. The Punica granatum chloroform extract (PGCE) and Punica grantum methanol extract (PGME) orally at 100, 200 and 400mg/kg, respectively, were administered along with ethylene glycol for 28 days. On 28 day, 24h urine was collected from individual rats and used for estimation of urine calcium, phosphate and oxalate. The serum creatinine, urea and uric acid levels were estimated in each animal. The kidney homogenate was used for the estimation of renal oxalate contents. The paraffin kidney sections were prepared to observe the CaOx deposits.
The ethylene glycol control (Gr.-II) had significant (P |
| doi_str_mv | 10.1016/j.jep.2012.01.003 |
| format | article |
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The traditional use of Punica granatum has been reported to regulate urine discharge and controls the burning sensation of urine.
Animals model of calcium oxalate urolithiasis was developed in male rats by adding ethylene glycol 0.75% in drinking water. The Punica granatum chloroform extract (PGCE) and Punica grantum methanol extract (PGME) orally at 100, 200 and 400mg/kg, respectively, were administered along with ethylene glycol for 28 days. On 28 day, 24h urine was collected from individual rats and used for estimation of urine calcium, phosphate and oxalate. The serum creatinine, urea and uric acid levels were estimated in each animal. The kidney homogenate was used for the estimation of renal oxalate contents. The paraffin kidney sections were prepared to observe the CaOx deposits.
The ethylene glycol control (Gr.-II) had significant (P<0.001 vs. normal) increase in levels of urine oxalate, calcium and phosphate, serum creatinine, urea and uric acid and renal tissues oxalates, as compared to normal (Gr.-I). The paraffin kidney sections show significant histopathological changes. The treatment of PGCE and PGME at 100, 200 and 400mg/kg doses, significantly (P<0.001 vs. control) decreased the urine oxalate, calcium and phosphate, renal tissue oxalates and serum creatinine, urea and uric acid, in EG induced urolithiasis after 28 days.
The PGCE and PGME at the doses of 400mg/kg, found to be more effective in decreasing the urolithiasis and regeneration of renal tissues in male rats.</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2012.01.003</identifier><identifier>PMID: 22285521</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>alkanes ; animal models ; Animals ; calcium ; Calcium - urine ; calcium oxalate ; Calcium Oxalate - urine ; chloroform ; creatinine ; Creatinine - blood ; Cystone ; drinking water ; Ethylene Glycol ; histopathology ; Kidney - drug effects ; Kidney - metabolism ; Kidney - pathology ; Kidney Calculi - metabolism ; Kidney Calculi - pathology ; Kidney Calculi - prevention & control ; kidneys ; Male ; methanol ; Oxalates - metabolism ; Phosphates - urine ; Phytotherapy ; Plant Extracts - pharmacology ; Plant Extracts - therapeutic use ; prescribed burning ; Punica granatum ; Punicaceae ; Rats ; Rats, Wistar ; sensation ; tissue repair ; urea ; Urea - blood ; uric acid ; Uric Acid - blood ; urine ; Urolithiasis ; Urolithiasis - metabolism ; Urolithiasis - pathology ; Urolithiasis - prevention & control</subject><ispartof>Journal of ethnopharmacology, 2012-03, Vol.140 (2), p.234-238</ispartof><rights>2012 Elsevier Ireland Ltd</rights><rights>Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-c988ca78d7eeae2f038ac3b2fc20eb639c5f76e67d9d39222c4bb58e26fe164a3</citedby><cites>FETCH-LOGICAL-c376t-c988ca78d7eeae2f038ac3b2fc20eb639c5f76e67d9d39222c4bb58e26fe164a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22285521$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rathod, N.R.</creatorcontrib><creatorcontrib>Biswas, Dipak</creatorcontrib><creatorcontrib>Chitme, H.R.</creatorcontrib><creatorcontrib>Ratna, Sanjeev</creatorcontrib><creatorcontrib>Muchandi, I.S.</creatorcontrib><creatorcontrib>Chandra, Ramesh</creatorcontrib><title>Anti-urolithiatic effects of Punica granatum in male rats</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>In Ayurvedic medicine, the Punica granatum is considered “a Pharmacy unto itself”. In the present study we found that the chloroform and methanol extract of Punica granatum fruits has a role in preventing Urolithiasis in male rats.
The traditional use of Punica granatum has been reported to regulate urine discharge and controls the burning sensation of urine.
Animals model of calcium oxalate urolithiasis was developed in male rats by adding ethylene glycol 0.75% in drinking water. The Punica granatum chloroform extract (PGCE) and Punica grantum methanol extract (PGME) orally at 100, 200 and 400mg/kg, respectively, were administered along with ethylene glycol for 28 days. On 28 day, 24h urine was collected from individual rats and used for estimation of urine calcium, phosphate and oxalate. The serum creatinine, urea and uric acid levels were estimated in each animal. The kidney homogenate was used for the estimation of renal oxalate contents. The paraffin kidney sections were prepared to observe the CaOx deposits.
The ethylene glycol control (Gr.-II) had significant (P<0.001 vs. normal) increase in levels of urine oxalate, calcium and phosphate, serum creatinine, urea and uric acid and renal tissues oxalates, as compared to normal (Gr.-I). The paraffin kidney sections show significant histopathological changes. The treatment of PGCE and PGME at 100, 200 and 400mg/kg doses, significantly (P<0.001 vs. control) decreased the urine oxalate, calcium and phosphate, renal tissue oxalates and serum creatinine, urea and uric acid, in EG induced urolithiasis after 28 days.
The PGCE and PGME at the doses of 400mg/kg, found to be more effective in decreasing the urolithiasis and regeneration of renal tissues in male rats.</description><subject>alkanes</subject><subject>animal models</subject><subject>Animals</subject><subject>calcium</subject><subject>Calcium - urine</subject><subject>calcium oxalate</subject><subject>Calcium Oxalate - urine</subject><subject>chloroform</subject><subject>creatinine</subject><subject>Creatinine - blood</subject><subject>Cystone</subject><subject>drinking water</subject><subject>Ethylene Glycol</subject><subject>histopathology</subject><subject>Kidney - drug effects</subject><subject>Kidney - metabolism</subject><subject>Kidney - pathology</subject><subject>Kidney Calculi - metabolism</subject><subject>Kidney Calculi - pathology</subject><subject>Kidney Calculi - prevention & control</subject><subject>kidneys</subject><subject>Male</subject><subject>methanol</subject><subject>Oxalates - metabolism</subject><subject>Phosphates - urine</subject><subject>Phytotherapy</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - therapeutic use</subject><subject>prescribed burning</subject><subject>Punica granatum</subject><subject>Punicaceae</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>sensation</subject><subject>tissue repair</subject><subject>urea</subject><subject>Urea - blood</subject><subject>uric acid</subject><subject>Uric Acid - blood</subject><subject>urine</subject><subject>Urolithiasis</subject><subject>Urolithiasis - metabolism</subject><subject>Urolithiasis - pathology</subject><subject>Urolithiasis - prevention & control</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLxDAUhYMozjj6A9xod65ab5K2SXEl4gsGFNR1SNMbzdDHmLSC_97ojC5d3c13zj18hBxTyCjQ8nyVrXCdMaAsA5oB8B0yp1KwVBSC75I5cCFTKXI6IwchrABA0Bz2yYwxJouC0TmpLvvRpZMfWje-OT06k6C1aMaQDDZ5nHpndPLqda_HqUtcn3S6xcTrMRySPavbgEfbuyAvN9fPV3fp8uH2_upymRouyjE1lZRGC9kIRI3MApfa8JpZwwDrklemsKLEUjRVw6s4zOR1XUhkpUVa5povyNmmd-2H9wnDqDoXDLat7nGYgqqYKCsKgkWSbkjjhxA8WrX2rtP-U1FQ38LUSkVh6luYAqqisJg52bZPdYfNX-LXUARON4DVg9Kv3gX18hQbcoiFXPy8vdgQGC18OPQqGIe9wcb5KFI1g_tnwBfbp4Qv</recordid><startdate>20120327</startdate><enddate>20120327</enddate><creator>Rathod, N.R.</creator><creator>Biswas, Dipak</creator><creator>Chitme, H.R.</creator><creator>Ratna, Sanjeev</creator><creator>Muchandi, I.S.</creator><creator>Chandra, Ramesh</creator><general>Elsevier Ireland Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120327</creationdate><title>Anti-urolithiatic effects of Punica granatum in male rats</title><author>Rathod, N.R. ; Biswas, Dipak ; Chitme, H.R. ; Ratna, Sanjeev ; Muchandi, I.S. ; Chandra, Ramesh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-c988ca78d7eeae2f038ac3b2fc20eb639c5f76e67d9d39222c4bb58e26fe164a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>alkanes</topic><topic>animal models</topic><topic>Animals</topic><topic>calcium</topic><topic>Calcium - urine</topic><topic>calcium oxalate</topic><topic>Calcium Oxalate - urine</topic><topic>chloroform</topic><topic>creatinine</topic><topic>Creatinine - blood</topic><topic>Cystone</topic><topic>drinking water</topic><topic>Ethylene Glycol</topic><topic>histopathology</topic><topic>Kidney - drug effects</topic><topic>Kidney - metabolism</topic><topic>Kidney - pathology</topic><topic>Kidney Calculi - metabolism</topic><topic>Kidney Calculi - pathology</topic><topic>Kidney Calculi - prevention & control</topic><topic>kidneys</topic><topic>Male</topic><topic>methanol</topic><topic>Oxalates - metabolism</topic><topic>Phosphates - urine</topic><topic>Phytotherapy</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Extracts - therapeutic use</topic><topic>prescribed burning</topic><topic>Punica granatum</topic><topic>Punicaceae</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>sensation</topic><topic>tissue repair</topic><topic>urea</topic><topic>Urea - blood</topic><topic>uric acid</topic><topic>Uric Acid - blood</topic><topic>urine</topic><topic>Urolithiasis</topic><topic>Urolithiasis - metabolism</topic><topic>Urolithiasis - pathology</topic><topic>Urolithiasis - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rathod, N.R.</creatorcontrib><creatorcontrib>Biswas, Dipak</creatorcontrib><creatorcontrib>Chitme, H.R.</creatorcontrib><creatorcontrib>Ratna, Sanjeev</creatorcontrib><creatorcontrib>Muchandi, I.S.</creatorcontrib><creatorcontrib>Chandra, Ramesh</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rathod, N.R.</au><au>Biswas, Dipak</au><au>Chitme, H.R.</au><au>Ratna, Sanjeev</au><au>Muchandi, I.S.</au><au>Chandra, Ramesh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-urolithiatic effects of Punica granatum in male rats</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2012-03-27</date><risdate>2012</risdate><volume>140</volume><issue>2</issue><spage>234</spage><epage>238</epage><pages>234-238</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>In Ayurvedic medicine, the Punica granatum is considered “a Pharmacy unto itself”. In the present study we found that the chloroform and methanol extract of Punica granatum fruits has a role in preventing Urolithiasis in male rats.
The traditional use of Punica granatum has been reported to regulate urine discharge and controls the burning sensation of urine.
Animals model of calcium oxalate urolithiasis was developed in male rats by adding ethylene glycol 0.75% in drinking water. The Punica granatum chloroform extract (PGCE) and Punica grantum methanol extract (PGME) orally at 100, 200 and 400mg/kg, respectively, were administered along with ethylene glycol for 28 days. On 28 day, 24h urine was collected from individual rats and used for estimation of urine calcium, phosphate and oxalate. The serum creatinine, urea and uric acid levels were estimated in each animal. The kidney homogenate was used for the estimation of renal oxalate contents. The paraffin kidney sections were prepared to observe the CaOx deposits.
The ethylene glycol control (Gr.-II) had significant (P<0.001 vs. normal) increase in levels of urine oxalate, calcium and phosphate, serum creatinine, urea and uric acid and renal tissues oxalates, as compared to normal (Gr.-I). The paraffin kidney sections show significant histopathological changes. The treatment of PGCE and PGME at 100, 200 and 400mg/kg doses, significantly (P<0.001 vs. control) decreased the urine oxalate, calcium and phosphate, renal tissue oxalates and serum creatinine, urea and uric acid, in EG induced urolithiasis after 28 days.
The PGCE and PGME at the doses of 400mg/kg, found to be more effective in decreasing the urolithiasis and regeneration of renal tissues in male rats.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>22285521</pmid><doi>10.1016/j.jep.2012.01.003</doi><tpages>5</tpages></addata></record> |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | alkanes animal models Animals calcium Calcium - urine calcium oxalate Calcium Oxalate - urine chloroform creatinine Creatinine - blood Cystone drinking water Ethylene Glycol histopathology Kidney - drug effects Kidney - metabolism Kidney - pathology Kidney Calculi - metabolism Kidney Calculi - pathology Kidney Calculi - prevention & control kidneys Male methanol Oxalates - metabolism Phosphates - urine Phytotherapy Plant Extracts - pharmacology Plant Extracts - therapeutic use prescribed burning Punica granatum Punicaceae Rats Rats, Wistar sensation tissue repair urea Urea - blood uric acid Uric Acid - blood urine Urolithiasis Urolithiasis - metabolism Urolithiasis - pathology Urolithiasis - prevention & control |
| title | Anti-urolithiatic effects of Punica granatum in male rats |
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