Separation and Identification of Volatile Compounds from Liquid Cultures of Trichoderma harzianum by GC-MS using Three Different Capillary Columns

A simple, fast, repeatable and less laborious sample preparation protocol was developed and applied for the analysis of biocontrol fungus Trichoderma harzianum strain FA1132 by using gas chromatography-mass spectrometry. The match factors for sample spectra with respect to the mass spectra library o...

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Bibliographic Details
Published in:Journal of chromatographic science 2012-04, Vol.50 (4), p.358-367
Main Authors: Siddiquee, Shafiquzzaman, Cheong, Bo Eng, Taslima, Khanam, Kausar, Hossain, Hasan, Md Mainul
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Bioreactors
Capillarity
Chemistry
Chromatographic methods and physical methods associated with chromatography
Culture
Culture Media, Conditioned
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Fungi
Gas Chromatography-Mass Spectrometry - methods
Hydrocarbons
Mass spectrometry
Methanol
Microbiology
Morphology, structure, chemical composition
Mycology
Spectra
Trichoderma - metabolism
Trichoderma harzianum
Volatile compounds
Volatile Organic Compounds - analysis
Volatile Organic Compounds - isolation & purification
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Summary:A simple, fast, repeatable and less laborious sample preparation protocol was developed and applied for the analysis of biocontrol fungus Trichoderma harzianum strain FA1132 by using gas chromatography-mass spectrometry. The match factors for sample spectra with respect to the mass spectra library of fungal volatile compounds were determined and used to study the complex hydrocarbons and other volatile compounds, which were separated by using different capillary columns with nonpolar, medium polar and high polar stationary phases. To date, more than 278 volatile compounds (with spectral match factor at least 90%) such as normal saturated hydrocarbons (C7-C30), cyclohexane, cyclopentane, fatty acids, alcohols, esters, sulfur-containing compounds, simple pyrane and benzene derivatives have been identified. Most of these compounds have not previously been reported. The method described in this paper is a more convenient research tool for the detection of volatile compounds from the cultures of T. harzianum.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bms012