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Cholesterol efflux from macrophages is influenced differentially by plasmas from overweight insulin-sensitive and -resistant subjects
Objective: In vitro measurements of cholesterol efflux from macrophages have recently been shown to associate with cardiovascular risk. We investigated whether cholesterol efflux from macrophages incubated with plasmas from overweight/obese subjects with metabolic syndrome was influenced by the pres...
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| Published in: | International Journal of Obesity 2012-03, Vol.36 (3), p.407-413 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c607t-37580225d06554b39e1ba92efc35d064cdc577d91b5397326b4e778020f836623 |
| container_end_page | 413 |
| container_issue | 3 |
| container_start_page | 407 |
| container_title | International Journal of Obesity |
| container_volume | 36 |
| creator | Nestel, P Hoang, A Sviridov, D Straznicky, N |
| description | Objective:
In vitro
measurements of cholesterol efflux from macrophages have recently been shown to associate with cardiovascular risk. We investigated whether cholesterol efflux from macrophages incubated with plasmas from overweight/obese subjects with metabolic syndrome was influenced by the presence of insulin resistance.
Methods:
Plasmas were obtained from 47 men and women with metabolic syndrome, of whom 25 were found to be insulin resistant (IR) and 22 insulin sensitive (IS) (Matsuda, De Fronzo equation based on oral glucose tolerance test). Activated human macrophage THP-1 cells in which cholesterol had been radiolabelled were incubated with the subjects’ plasmas to allow calculation of % cholesterol efflux.
Results:
Body mass index and waist measurements, as well as plasma lipid levels, did not differ between the two groups. Homeostatic model assessment-insulin resistance value as well as plasma insulin and leptin concentrations were higher in IR subjects. Cholesterol efflux was found to be significantly greater with plasmas from IR subjects (9.1%) than from IS subjects (6.7%) (
P
=0.005). Further, cholesterol efflux was significantly inversely associated with insulin sensitivity index (
P |
| doi_str_mv | 10.1038/ijo.2011.170 |
| format | article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_927989331</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A284323960</galeid><sourcerecordid>A284323960</sourcerecordid><originalsourceid>FETCH-LOGICAL-c607t-37580225d06554b39e1ba92efc35d064cdc577d91b5397326b4e778020f836623</originalsourceid><addsrcrecordid>eNp90kuP0zAQAOAIgdiycOOMIhCPAyl-xHF8XFW8pJW4wDlynHHryrGL7Sz0B_C_cZSyyyJAsRTJ_maSGU9RPMZojRFt35i9XxOE8RpzdKdY4Zo3FasFv1usEEW8QqxhZ8WDGPcIIcYQuV-cEdzyhtV8VfzY7LyFmCB4W4LWdvpe6uDHcpQq-MNObiGWJi-Xj8ApGMrBaA0BXDLS2mPZH8uDlXGUcQn0VxC-gdnuUg6KkzWuiuCiSeYKSumGsgoQTUzSpTJO_R5Uig-Le1raCI9O7_Piy7u3nzcfqstP7z9uLi4r1SCeKspZiwhhA2oYq3sqAPdSENCKznu1GhTjfBC4Z1RwSpq-Bs5zCNItbRpCz4uXS95D8F-nXHY3mqjAWunAT7EThItWUIqzfPVfiRFqW9Lk_8j06R9076fgch1zPo6xaGf0bEFbaaHL3fQpSDXn7C5IW1NCRYOyWv9F5WeA0SjvQJu8fyvgxW8BO5A27aK3UzLexdvw9QLzrcYYQHeHYEYZjrmSbp6jLs9RN89Rl-co8yenmqZ-hOEa_xqcDJ6fgIxKWh2kUybeOJZbI7DIrlpczEduC-GmOf_4cLl4J9MU4DphRrOZyU9YSOis</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>927711985</pqid></control><display><type>article</type><title>Cholesterol efflux from macrophages is influenced differentially by plasmas from overweight insulin-sensitive and -resistant subjects</title><source>Nature</source><creator>Nestel, P ; Hoang, A ; Sviridov, D ; Straznicky, N</creator><creatorcontrib>Nestel, P ; Hoang, A ; Sviridov, D ; Straznicky, N</creatorcontrib><description>Objective:
In vitro
measurements of cholesterol efflux from macrophages have recently been shown to associate with cardiovascular risk. We investigated whether cholesterol efflux from macrophages incubated with plasmas from overweight/obese subjects with metabolic syndrome was influenced by the presence of insulin resistance.
Methods:
Plasmas were obtained from 47 men and women with metabolic syndrome, of whom 25 were found to be insulin resistant (IR) and 22 insulin sensitive (IS) (Matsuda, De Fronzo equation based on oral glucose tolerance test). Activated human macrophage THP-1 cells in which cholesterol had been radiolabelled were incubated with the subjects’ plasmas to allow calculation of % cholesterol efflux.
Results:
Body mass index and waist measurements, as well as plasma lipid levels, did not differ between the two groups. Homeostatic model assessment-insulin resistance value as well as plasma insulin and leptin concentrations were higher in IR subjects. Cholesterol efflux was found to be significantly greater with plasmas from IR subjects (9.1%) than from IS subjects (6.7%) (
P
=0.005). Further, cholesterol efflux was significantly inversely associated with insulin sensitivity index (
P
<0.001), directly with arterial insulin concentration (
P
<0.001) and directly with cholesteryl ester transfer protein (CETP) mass (
P
=0.044).
Conclusion:
Plasmas from overweight subjects with insulin resistance induced greater
in vitro
cholesterol efflux compared with IS subjects. Efflux inversely correlated with insulin sensitivity suggesting an increase in reverse cholesterol transport in the IR state that may lead to greater transfer of cholesterol to apoB lipoproteins from high-density lipoproteins via CETP as a factor in the association between IR and atherosclerosis.</description><identifier>ISSN: 0307-0565</identifier><identifier>EISSN: 1476-5497</identifier><identifier>DOI: 10.1038/ijo.2011.170</identifier><identifier>PMID: 21876547</identifier><identifier>CODEN: IJOBDP</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Analysis ; Apolipoproteins ; Arteriosclerosis ; Atherosclerosis ; ATP Binding Cassette Transporter 1 ; ATP-Binding Cassette Transporters - metabolism ; Australia - epidemiology ; Biological and medical sciences ; Biological Transport ; Blood cholesterol ; Blood diseases ; Body mass index ; Body weight ; Cardiorespiratory ; Cardiovascular disease ; Cardiovascular diseases ; Cholesterol ; Cholesterol - metabolism ; Cholesterol Ester Transfer Proteins - metabolism ; Cholesteryl ester transfer protein ; Coronary Artery Disease - metabolism ; Coronary vessels ; Diabetes ; Disorders of blood lipids. Hyperlipoproteinemia ; Epidemiology ; Female ; Glucose tolerance ; Glucose Tolerance Test ; Health aspects ; Health Promotion and Disease Prevention ; Health risks ; High density lipoprotein ; Hormones ; Human subjects ; Humans ; Insulin ; Insulin - blood ; Insulin Resistance ; Internal Medicine ; Leptin ; Leptin - blood ; Lipids ; Lipoproteins ; Macrophages ; Macrophages - metabolism ; Male ; Mathematical models ; Measurement ; Medical sciences ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Metabolic disorders ; Metabolic syndrome ; Metabolic Syndrome - complications ; Metabolic Syndrome - epidemiology ; Metabolic Syndrome - metabolism ; Middle Aged ; Miscellaneous ; Obesity ; Obesity - complications ; Obesity - epidemiology ; Obesity - metabolism ; original-article ; Other metabolic disorders ; Overweight ; Overweight persons ; Physiological aspects ; Plasma ; Public Health ; Risk Factors ; Vein & artery diseases ; Women</subject><ispartof>International Journal of Obesity, 2012-03, Vol.36 (3), p.407-413</ispartof><rights>Macmillan Publishers Limited 2012</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2012 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Mar 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c607t-37580225d06554b39e1ba92efc35d064cdc577d91b5397326b4e778020f836623</citedby><cites>FETCH-LOGICAL-c607t-37580225d06554b39e1ba92efc35d064cdc577d91b5397326b4e778020f836623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2725,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25665919$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21876547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nestel, P</creatorcontrib><creatorcontrib>Hoang, A</creatorcontrib><creatorcontrib>Sviridov, D</creatorcontrib><creatorcontrib>Straznicky, N</creatorcontrib><title>Cholesterol efflux from macrophages is influenced differentially by plasmas from overweight insulin-sensitive and -resistant subjects</title><title>International Journal of Obesity</title><addtitle>Int J Obes</addtitle><addtitle>Int J Obes (Lond)</addtitle><description>Objective:
In vitro
measurements of cholesterol efflux from macrophages have recently been shown to associate with cardiovascular risk. We investigated whether cholesterol efflux from macrophages incubated with plasmas from overweight/obese subjects with metabolic syndrome was influenced by the presence of insulin resistance.
Methods:
Plasmas were obtained from 47 men and women with metabolic syndrome, of whom 25 were found to be insulin resistant (IR) and 22 insulin sensitive (IS) (Matsuda, De Fronzo equation based on oral glucose tolerance test). Activated human macrophage THP-1 cells in which cholesterol had been radiolabelled were incubated with the subjects’ plasmas to allow calculation of % cholesterol efflux.
Results:
Body mass index and waist measurements, as well as plasma lipid levels, did not differ between the two groups. Homeostatic model assessment-insulin resistance value as well as plasma insulin and leptin concentrations were higher in IR subjects. Cholesterol efflux was found to be significantly greater with plasmas from IR subjects (9.1%) than from IS subjects (6.7%) (
P
=0.005). Further, cholesterol efflux was significantly inversely associated with insulin sensitivity index (
P
<0.001), directly with arterial insulin concentration (
P
<0.001) and directly with cholesteryl ester transfer protein (CETP) mass (
P
=0.044).
Conclusion:
Plasmas from overweight subjects with insulin resistance induced greater
in vitro
cholesterol efflux compared with IS subjects. Efflux inversely correlated with insulin sensitivity suggesting an increase in reverse cholesterol transport in the IR state that may lead to greater transfer of cholesterol to apoB lipoproteins from high-density lipoproteins via CETP as a factor in the association between IR and atherosclerosis.</description><subject>Analysis</subject><subject>Apolipoproteins</subject><subject>Arteriosclerosis</subject><subject>Atherosclerosis</subject><subject>ATP Binding Cassette Transporter 1</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>Australia - epidemiology</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Blood cholesterol</subject><subject>Blood diseases</subject><subject>Body mass index</subject><subject>Body weight</subject><subject>Cardiorespiratory</subject><subject>Cardiovascular disease</subject><subject>Cardiovascular diseases</subject><subject>Cholesterol</subject><subject>Cholesterol - metabolism</subject><subject>Cholesterol Ester Transfer Proteins - metabolism</subject><subject>Cholesteryl ester transfer protein</subject><subject>Coronary Artery Disease - metabolism</subject><subject>Coronary vessels</subject><subject>Diabetes</subject><subject>Disorders of blood lipids. Hyperlipoproteinemia</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Glucose tolerance</subject><subject>Glucose Tolerance Test</subject><subject>Health aspects</subject><subject>Health Promotion and Disease Prevention</subject><subject>Health risks</subject><subject>High density lipoprotein</subject><subject>Hormones</subject><subject>Human subjects</subject><subject>Humans</subject><subject>Insulin</subject><subject>Insulin - blood</subject><subject>Insulin Resistance</subject><subject>Internal Medicine</subject><subject>Leptin</subject><subject>Leptin - blood</subject><subject>Lipids</subject><subject>Lipoproteins</subject><subject>Macrophages</subject><subject>Macrophages - metabolism</subject><subject>Male</subject><subject>Mathematical models</subject><subject>Measurement</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Metabolic disorders</subject><subject>Metabolic syndrome</subject><subject>Metabolic Syndrome - complications</subject><subject>Metabolic Syndrome - epidemiology</subject><subject>Metabolic Syndrome - metabolism</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Obesity</subject><subject>Obesity - complications</subject><subject>Obesity - epidemiology</subject><subject>Obesity - metabolism</subject><subject>original-article</subject><subject>Other metabolic disorders</subject><subject>Overweight</subject><subject>Overweight persons</subject><subject>Physiological aspects</subject><subject>Plasma</subject><subject>Public Health</subject><subject>Risk Factors</subject><subject>Vein & artery diseases</subject><subject>Women</subject><issn>0307-0565</issn><issn>1476-5497</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp90kuP0zAQAOAIgdiycOOMIhCPAyl-xHF8XFW8pJW4wDlynHHryrGL7Sz0B_C_cZSyyyJAsRTJ_maSGU9RPMZojRFt35i9XxOE8RpzdKdY4Zo3FasFv1usEEW8QqxhZ8WDGPcIIcYQuV-cEdzyhtV8VfzY7LyFmCB4W4LWdvpe6uDHcpQq-MNObiGWJi-Xj8ApGMrBaA0BXDLS2mPZH8uDlXGUcQn0VxC-gdnuUg6KkzWuiuCiSeYKSumGsgoQTUzSpTJO_R5Uig-Le1raCI9O7_Piy7u3nzcfqstP7z9uLi4r1SCeKspZiwhhA2oYq3sqAPdSENCKznu1GhTjfBC4Z1RwSpq-Bs5zCNItbRpCz4uXS95D8F-nXHY3mqjAWunAT7EThItWUIqzfPVfiRFqW9Lk_8j06R9076fgch1zPo6xaGf0bEFbaaHL3fQpSDXn7C5IW1NCRYOyWv9F5WeA0SjvQJu8fyvgxW8BO5A27aK3UzLexdvw9QLzrcYYQHeHYEYZjrmSbp6jLs9RN89Rl-co8yenmqZ-hOEa_xqcDJ6fgIxKWh2kUybeOJZbI7DIrlpczEduC-GmOf_4cLl4J9MU4DphRrOZyU9YSOis</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Nestel, P</creator><creator>Hoang, A</creator><creator>Sviridov, D</creator><creator>Straznicky, N</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T2</scope><scope>7TK</scope><scope>7TS</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Cholesterol efflux from macrophages is influenced differentially by plasmas from overweight insulin-sensitive and -resistant subjects</title><author>Nestel, P ; Hoang, A ; Sviridov, D ; Straznicky, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c607t-37580225d06554b39e1ba92efc35d064cdc577d91b5397326b4e778020f836623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analysis</topic><topic>Apolipoproteins</topic><topic>Arteriosclerosis</topic><topic>Atherosclerosis</topic><topic>ATP Binding Cassette Transporter 1</topic><topic>ATP-Binding Cassette Transporters - metabolism</topic><topic>Australia - epidemiology</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Blood cholesterol</topic><topic>Blood diseases</topic><topic>Body mass index</topic><topic>Body weight</topic><topic>Cardiorespiratory</topic><topic>Cardiovascular disease</topic><topic>Cardiovascular diseases</topic><topic>Cholesterol</topic><topic>Cholesterol - metabolism</topic><topic>Cholesterol Ester Transfer Proteins - metabolism</topic><topic>Cholesteryl ester transfer protein</topic><topic>Coronary Artery Disease - metabolism</topic><topic>Coronary vessels</topic><topic>Diabetes</topic><topic>Disorders of blood lipids. Hyperlipoproteinemia</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Glucose tolerance</topic><topic>Glucose Tolerance Test</topic><topic>Health aspects</topic><topic>Health Promotion and Disease Prevention</topic><topic>Health risks</topic><topic>High density lipoprotein</topic><topic>Hormones</topic><topic>Human subjects</topic><topic>Humans</topic><topic>Insulin</topic><topic>Insulin - blood</topic><topic>Insulin Resistance</topic><topic>Internal Medicine</topic><topic>Leptin</topic><topic>Leptin - blood</topic><topic>Lipids</topic><topic>Lipoproteins</topic><topic>Macrophages</topic><topic>Macrophages - metabolism</topic><topic>Male</topic><topic>Mathematical models</topic><topic>Measurement</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Metabolic disorders</topic><topic>Metabolic syndrome</topic><topic>Metabolic Syndrome - complications</topic><topic>Metabolic Syndrome - epidemiology</topic><topic>Metabolic Syndrome - metabolism</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Obesity</topic><topic>Obesity - complications</topic><topic>Obesity - epidemiology</topic><topic>Obesity - metabolism</topic><topic>original-article</topic><topic>Other metabolic disorders</topic><topic>Overweight</topic><topic>Overweight persons</topic><topic>Physiological aspects</topic><topic>Plasma</topic><topic>Public Health</topic><topic>Risk Factors</topic><topic>Vein & artery diseases</topic><topic>Women</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nestel, P</creatorcontrib><creatorcontrib>Hoang, A</creatorcontrib><creatorcontrib>Sviridov, D</creatorcontrib><creatorcontrib>Straznicky, N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Agricultural Science Collection</collection><collection>ProQuest - Health & Medical Complete保健、医学与药学数据库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Psychology Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International Journal of Obesity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nestel, P</au><au>Hoang, A</au><au>Sviridov, D</au><au>Straznicky, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cholesterol efflux from macrophages is influenced differentially by plasmas from overweight insulin-sensitive and -resistant subjects</atitle><jtitle>International Journal of Obesity</jtitle><stitle>Int J Obes</stitle><addtitle>Int J Obes (Lond)</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>36</volume><issue>3</issue><spage>407</spage><epage>413</epage><pages>407-413</pages><issn>0307-0565</issn><eissn>1476-5497</eissn><coden>IJOBDP</coden><abstract>Objective:
In vitro
measurements of cholesterol efflux from macrophages have recently been shown to associate with cardiovascular risk. We investigated whether cholesterol efflux from macrophages incubated with plasmas from overweight/obese subjects with metabolic syndrome was influenced by the presence of insulin resistance.
Methods:
Plasmas were obtained from 47 men and women with metabolic syndrome, of whom 25 were found to be insulin resistant (IR) and 22 insulin sensitive (IS) (Matsuda, De Fronzo equation based on oral glucose tolerance test). Activated human macrophage THP-1 cells in which cholesterol had been radiolabelled were incubated with the subjects’ plasmas to allow calculation of % cholesterol efflux.
Results:
Body mass index and waist measurements, as well as plasma lipid levels, did not differ between the two groups. Homeostatic model assessment-insulin resistance value as well as plasma insulin and leptin concentrations were higher in IR subjects. Cholesterol efflux was found to be significantly greater with plasmas from IR subjects (9.1%) than from IS subjects (6.7%) (
P
=0.005). Further, cholesterol efflux was significantly inversely associated with insulin sensitivity index (
P
<0.001), directly with arterial insulin concentration (
P
<0.001) and directly with cholesteryl ester transfer protein (CETP) mass (
P
=0.044).
Conclusion:
Plasmas from overweight subjects with insulin resistance induced greater
in vitro
cholesterol efflux compared with IS subjects. Efflux inversely correlated with insulin sensitivity suggesting an increase in reverse cholesterol transport in the IR state that may lead to greater transfer of cholesterol to apoB lipoproteins from high-density lipoproteins via CETP as a factor in the association between IR and atherosclerosis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>21876547</pmid><doi>10.1038/ijo.2011.170</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0307-0565 |
| ispartof | International Journal of Obesity, 2012-03, Vol.36 (3), p.407-413 |
| issn | 0307-0565 1476-5497 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_927989331 |
| source | Nature |
| subjects | Analysis Apolipoproteins Arteriosclerosis Atherosclerosis ATP Binding Cassette Transporter 1 ATP-Binding Cassette Transporters - metabolism Australia - epidemiology Biological and medical sciences Biological Transport Blood cholesterol Blood diseases Body mass index Body weight Cardiorespiratory Cardiovascular disease Cardiovascular diseases Cholesterol Cholesterol - metabolism Cholesterol Ester Transfer Proteins - metabolism Cholesteryl ester transfer protein Coronary Artery Disease - metabolism Coronary vessels Diabetes Disorders of blood lipids. Hyperlipoproteinemia Epidemiology Female Glucose tolerance Glucose Tolerance Test Health aspects Health Promotion and Disease Prevention Health risks High density lipoprotein Hormones Human subjects Humans Insulin Insulin - blood Insulin Resistance Internal Medicine Leptin Leptin - blood Lipids Lipoproteins Macrophages Macrophages - metabolism Male Mathematical models Measurement Medical sciences Medicine Medicine & Public Health Metabolic Diseases Metabolic disorders Metabolic syndrome Metabolic Syndrome - complications Metabolic Syndrome - epidemiology Metabolic Syndrome - metabolism Middle Aged Miscellaneous Obesity Obesity - complications Obesity - epidemiology Obesity - metabolism original-article Other metabolic disorders Overweight Overweight persons Physiological aspects Plasma Public Health Risk Factors Vein & artery diseases Women |
| title | Cholesterol efflux from macrophages is influenced differentially by plasmas from overweight insulin-sensitive and -resistant subjects |
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