Interaction of plectin and intermediate filaments

Abstract Background Plectin, a member of the plakin family proteins, is a high molecular weight protein that is ubiquitously expressed. It acts as a cytolinker for the three major components of the cyotoskeleton, namely actin microfilaments, microtubules and intermediate filaments. Objective The aim...

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Bibliographic Details
Published in:Journal of dermatological science 2012-04, Vol.66 (1), p.44-50
Main Authors: Karashima, Tadashi, Tsuruta, Daisuke, Hamada, Takahiro, Ishii, Norito, Ono, Fumitake, Hashikawa, Keiko, Ohyama, Bungo, Natsuaki, Yohei, Fukuda, Shunpei, Koga, Hiroshi, Sogame, Ryosuke, Nakama, Takekuni, Dainichi, Teruki, Hashimoto, Takashi
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - metabolism
Adenocarcinoma
Adrenal Gland Neoplasms
Animals
Cell Line, Tumor
Cercopithecus aethiops
COS Cells
Cross-Linking Reagents - metabolism
Dermatology
Gene Deletion
Intermediate filament
Intermediate Filaments - metabolism
Keratin
Keratinocytes - cytology
Keratinocytes - physiology
Keratins - metabolism
Plakin
Plectin
Plectin - chemistry
Plectin - genetics
Plectin - metabolism
Protein Binding - physiology
Protein Structure, Tertiary
Vimentin
Vimentin - genetics
Vimentin - metabolism
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Summary:Abstract Background Plectin, a member of the plakin family proteins, is a high molecular weight protein that is ubiquitously expressed. It acts as a cytolinker for the three major components of the cyotoskeleton, namely actin microfilaments, microtubules and intermediate filaments. Objective The aim of our experiments was to identify new binding sites for intermediate filaments on plectin and to specify these sites. Methods We introduced truncated forms of plectin into several cell lines and observe interaction between plectin and intermediate filaments. Results We found that a linker region in the COOH-terminal end of plectin was required for the association of the protein with intermediate filaments. In addition, we also demonstrated that a serine residue at position 4645 of plectin may have a role on binding of plectin to intermediate filaments. Conclusion A linker region in the COOH-terminal end and serine residue at position 4645 may be important for the binding of plectin to intermediate filaments.
ISSN:0923-1811
1873-569X
DOI:10.1016/j.jdermsci.2012.01.008