Feed-control development for succinic acid production with Anaerobiospirillum succiniciproducens

• Published in: Biotechnology and bioengineering 2012-05, Vol.109 (5), p.1187-1192
• Main Authors: Bretz, Karlheinz, Kabasci, Stephan
• Format: Article
• Language: English
• Subjects: Acids; Anaerobiospirillum - growth & development; Anaerobiospirillum - metabolism; Anaerobiospirillum succiniciproducens; Bioengineering; Biotechnology; Correlation; Cultivation; Culture Media - chemistry; fed-batch; Glucose; Glucose - metabolism; Gram-negative bacteria; Hydrogen-Ion Concentration; Metabolism; Models, Theoretical; Succinic acid; Succinic Acid - metabolism
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.24387

The aim of this work was the development of a feed‐control for a succinic acid production fed‐batch process. The performed batch trials indicated a correlation between succinic acid production and base consumption pH control. Based on the metabolism of Anaerobiospirillum succiniciproducens, a theoretical correlation between base consumption and glucose feed was established and proved in cultivation trials. With the established fed‐batch process, the succinic acid yield could be increased to 0.875 (g/g glucose) in comparison to batch processes (0.60) with similar glucose concentrations. Additionally, the results indicate that the osmolarity of the medium has a significant influence on succinic acid production. Biotechnol. Bioeng. 2012; 109:1187–1192. © 2011 Wiley Periodicals, Inc. Based on the metabolic pathway of Anaerobiospirillum succiniciproducens from glucose to succinic and acetic acid, the theoretical base consumption was calculated. The base pump signal during fermentation was used as a feed‐pump signal for an automated feed‐control. The proof of concept was validated by experiments.