The expression of genes encoding for COX-2, MPO, iNOS, and sPLA2-IIA in patients with recurrent depressive disorder

• Published in: Journal of affective disorders 2012-05, Vol.138 (3), p.360-366
• Main Authors: Gałecki, Piotr, Gałecka, Elżbieta, Maes, Michael, Chamielec, Marcelina, Orzechowska, Agata, Bobińska, Kinga, Lewiński, Andrzej, Szemraj, Janusz
• Format: Article
• Language: English
• Subjects: Adult; Adult and adolescent clinical studies; Antidepressive Agents - therapeutic use; Biological and medical sciences; Blood cells; Cyclooxygenase 2 - genetics; Cyclooxygenase-2; Depression; Depressive Disorder - drug therapy; Depressive Disorder - genetics; Depressive personality disorders; Encoding; Female; Gene Expression; Genes; Group II Phospholipases A2 - genetics; Humans; Inducible nitric oxide synthase; Male; Medical sciences; Middle Aged; Mood disorders; mRNA expression; Myeloperoxidase; Nitric Oxide Synthase Type II - genetics; Pathophysiological aspects; Peroxidase - genetics; Psychiatry; Psychology. Psychoanalysis. Psychiatry; Psychopathology. Psychiatry; Recurrence; RNA, Messenger - biosynthesis; Secretory phospholipase A2
• ISSN: 0165-0327; 1573-2517
• DOI: 10.1016/j.jad.2012.01.016

Abstract Background There is evidence that inflammation, oxidative and nitrosative stress (IO&NS) play a role in the pathophysiology of depression. There are also data indicating altered inflammatory gene expression in depressive disorder and that genetic variants of IO&NS genes are associated with increased risk of the disease in question. The aim of this study was to explore mRNA expression of four IO&NS genes PTGS2 , MPO , NOS2A , and PLA2G2A coding respectively: cyclooxygenase-2 (COX-2), myeloperoxidase (MPO), inducible nitric oxide synthase (iNOS) and secretory phospholipase A2 type IIA (sPLA2-IIA). Method Expression of the mRNA was determined using quantitative real-time PCR, in peripheral blood cells of patients with recurrent depressive disorder (rDD) and normal controls. Results The mRNA expressions of the genes encoding for COX-2, MPO, iNOS and sPLA2-IIA were significantly increased in the peripheral blood cells of depressed patients versus controls. Limitations Patients were treated with antidepressants. Conclusion Our results indicate and may confirm the role of peripheral IO&NS pathways in the pathophysiology of depression. The results represent a promising way to investigate biological markers of depression.