Evaluation of the matrix effect of thermophilic anaerobic digestion on inactivation of infectious laryngotracheitis virus using real-time PCR and viral cell culture

• Published in: Bioresource technology 2012-04, Vol.110, p.692-696
• Main Authors: Gao, Tiejun, Bowlby, Evelyn, Tong, Yupin, Wu, John T.Y., Wong, Lester, Tower, Robert J., Pang, Xiaoli, Li, Xiaomei
• Format: Article
• Language: English
• Subjects: Anaerobic digestion; Anaerobiosis; Biowaste; cell culture; Cell Line, Tumor; Cytopathogenic Effect, Viral; cytopathogenicity; DNA; DNA fragmentation; Gallid herpesvirus 1; Herpesvirus 1, Gallid - drug effects; Herpesvirus 1, Gallid - genetics; Herpesvirus 1, Gallid - pathogenicity; Humans; Inactivation; Infectious laryngotracheitis virus; polymerase chain reaction; Poultry; poultry carcasses; Real-Time Polymerase Chain Reaction - methods; Thermophilic; viruses
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2012.01.166

► True matrix effect of TAD on inactivation of ILT virus was explored first time. ► TAD matrix alone eliminates the viral infectivity and impairs the viral DNA core of ILT virus. ► Viral inactivation of TAD matrix was in the time- and dose-dependant manner. ► TAD process was a cost-effective and environmentally friendly way to treat poultry biowaste and dead chicken. The matrix effect of the thermophilic anaerobic digestion (TAD) process on inactivation of infectious laryngotracheitis virus (ILTV) was evaluated. Viral cell culture and real-time PCR were used for assessing removal of the viral infectivity and degradation of viral DNA, respectively. Results showed that the TAD-derived matrix alone can inactivate the virus and destroy the nucleic acid helix core of ILTV in a time-and- dose-dependent manner. No cytopathogenic effect (CPE) was observed in the cells exposed to ILTV pre-treated with TAD matrix for 1.5h in experiment 1 and for 16h in experiment 2. There was a significant statistical difference between TAD matrix treated and non-treated cultures (p