Ischemia modified albumin and carbonyl protein as potential biomarkers of protein oxidation in hemodialysis

• Published in: Clinical biochemistry 2012-04, Vol.45 (6), p.450-454
• Main Authors: Albarello, Kassiano, dos Santos, Gélson Antônio, Bochi, Guilherme Vargas, Sangoi, Manuela Borges, Almeida, Taís Corrêa, Paz da Silva, José Edson, Garcia, Solange Cristina, Moresco, Rafael Noal
• Format: Article
• Language: English
• Subjects: Adult; Aged; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; Biomarkers - blood; Chronic kidney disease; Emergency and intensive care: renal failure. Dialysis management; Female; Hemodialysis; Humans; Intensive care medicine; Investigative techniques, diagnostic techniques (general aspects); Ischemia modified albumin; Kidney Failure, Chronic - blood; Kidney Failure, Chronic - metabolism; Kidney Failure, Chronic - therapy; Kidneys; Male; Medical sciences; Middle Aged; Nephrology. Urinary tract diseases; Oxidation-Reduction; Oxidative Stress; Protein Carbonylation; Renal Dialysis; Serum Albumin - metabolism; Urinary system involvement in other diseases. Miscellaneous
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2012.01.031

The aim of this study was to evaluate the effect of HD on ischemia modified albumin (IMA) and protein carbonyl groups in order to investigate the role of IMA as a marker of protein oxidation. This study was conducted with 23 chronic hemodialysis patients. The serum IMA levels and protein carbonyl groups were measured immediately before hemodialysis (pre-HD) and after the end of hemodialysis (post-HD). IMA concentrations were significantly higher in post-HD than those of the pre-HD and carbonyl protein concentrations were higher in post-HD in comparison with pre-HD. A significant correlation was observed between IMA and carbonyl protein levels. The increase of IMA levels and protein carbonyl groups post-HD could be attributed to the increase of oxidative stress associated with HD, and IMA appears to be an important biomarker for assessing protein oxidation after HD. ► Effects of hemodialysis (HD) on ischemia modified albumin (IMA) and protein carbonyl groups. ► IMA and protein carbonyl as biomarkers of protein oxidation. ► IMA and carbonyl protein concentrations were higher in post-HD. ► HD is associated with overproduction of free radicals which may produce a chemical modification of proteins.