Evaluation of plasmatic MMP-8, MMP-9, TIMP-1 and MPO levels in obese and lean women

• Published in: Clinical biochemistry 2012-04, Vol.45 (6), p.412-415
• Main Authors: Andrade, Vanessa L., Petruceli, Eveline, Belo, Vanessa A., Andrade-Fernandes, Claudia M., Caetano Russi, Cristiane V., Bosco, Adriana Ap, Tanus-Santos, José Eduardo, Sandrim, Valeria Cristina
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Female; Humans; Investigative techniques, diagnostic techniques (general aspects); Matrix Metalloproteinase 8 - blood; Matrix Metalloproteinase 9 - blood; Medical sciences; Metabolic diseases; Middle Aged; MMP-8; MMP-9; MPO; Neutrophil activation; Neutrophil collagenase; Obesity; Obesity - blood; Peroxidase - blood; Thinness - blood; TIMP-1; Tissue Inhibitor of Metalloproteinase-1 - blood
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2012.01.008

To compare the plasma concentrations of matrix metalloproteinase (MMP)-9, tissue inhibitor of MMP (TIMP)-1, MMP-8, and myeloperoxidase (MPO) for obese and lean women. We recruited 30 lean and 36 obese women without comorbidities. The MMP-9, TIMP-1, and MMP-8 levels were measured using enzyme-linked immunosorbent assay (ELISA). MPO activity was assessed by a colorimetric assay. Obese women had higher MMP-9 levels and MMP-9:TIMP-1 ratios than lean women. Conversely, the MMP-8 levels and MMP-8:TIMP-1 ratios in the obese women were significantly lower than those in the lean women despite neutrophil activation, which was assessed by MPO activity. We observed that MMP-9 and MMP-8 had distinct profiles, which suggested that these 2 enzymes play different roles in obesity. ► We show increase and reduced levels of MMP-9 and MMP-8 comparing obese versus lean women. ► Neutrophil is activates in obesity. ► Suggest different biological meaning of these two enzymes.