Resveratrol regulates lipolysis via adipose triglyceride lipase

• Published in: The Journal of nutritional biochemistry 2012-04, Vol.23 (4), p.379-384
• Main Authors: Lasa, Arrate, Schweiger, Martina, Kotzbeck, Petra, Churruca, Itziar, Simón, Edurne, Zechner, Rudolf, Portillo, María del Puy
• Format: Article
• Language: English
• Subjects: 3T3-L1; 3T3-L1 Cells; adipocytes; Adipocytes - drug effects; Adipocytes - enzymology; Adipose Tissue, White - cytology; Adipose Tissue, White - drug effects; Adipose Tissue, White - enzymology; Adipose triglyceride lipase; AMP-activated protein kinase; AMP-Activated Protein Kinases - genetics; AMP-Activated Protein Kinases - metabolism; Animals; Biological and medical sciences; Cell Line; enzyme activation; fatty acids; Fatty Acids, Nonesterified - metabolism; Feeding. Feeding behavior; free fatty acids; Fundamental and applied biological sciences. Psychology; genes; glycerol; Hormone-sensitive lipase; Humans; Isoproterenol - metabolism; Lipase - genetics; Lipase - metabolism; lipolysis; Lipolysis - drug effects; Male; Mice; Mice, Knockout; protein synthesis; Resveratrol; Sterol Esterase - antagonists & inhibitors; Sterol Esterase - genetics; Sterol Esterase - metabolism; Stilbenes - pharmacology; triacylglycerol lipase; Triglycerides - analysis; Vertebrates: anatomy and physiology, studies on body, several organs or systems; white adipose tissue
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2010.12.014

Resveratrol has been reported to increase adrenaline-induced lipolysis in 3T3-L1 adipocytes. The general aim of the present work was to gain more insight concerning the effects of trans-resveratrol on lipid mobilization. The specific purpose was to assess the involvement of the two main lipases: adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), in the activation of lipolysis induced by this molecule. For lipolysis experiments, 3T3-L1 and human SGBS adipocytes as well as adipose tissue from wild-type, ATGL knockout and HSL knockout mice were used. Moreover, gene and protein expressions of these lipases were analyzed. Resveratrol-induced free fatty acids release but not glycerol release in 3T3-L1 under basal and isoproterenol-stimulating conditions and under isoproterenol-stimulating conditions in SGBS adipocytes. When HSL was blocked by compound 76-0079, free fatty acid release was still induced by resveratrol. By contrast, in the presence of the compound C, an inhibitor of adenosine monophosphate-activated protein kinase, resveratrol effect was totally blunted. Resveratrol increased ATGL gene and protein expressions, an effect that was not observed for HSL. Resveratrol increased fatty acids release in epididymal adipose tissue from wild-type and HSL knockout mice but not in that adipose tissue from ATGL knockout mice. Taking as a whole, the present results provide novel evidence that resveratrol regulates lipolytic activity in human and murine adipocytes, as well as in white adipose tissue from mice, acting mainly on ATGL at transcriptional and posttranscriptional levels. Enzyme activation seems to be induced via adenosine monophosphate-activated protein kinase.