Analysis of biomarkers characteristic of porcine liver injury—from biomolecular logic gates to an animal model

A biocatalytic cascade for the analysis of the simultaneous increase in the concentration of two biomarkers characteristic of liver injury (alanine transaminase, ALT, and lactate dehydrogenase, LDH) was tested on real samples acquired from an animal model (domestic pigs, Sus scrofa domesticus) suffe...

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Bibliographic Details
Published in:Analyst (London) 2012-04, Vol.137 (8), p.1768-1770
Main Authors: HALAMKOVA, Lenka, HALAMEK, Jan, BOCHAROVA, Vera, WOLF, Steven, MULIER, Kristine E, BEILMAN, Greg, WANG, Joseph, KATZ, Evgeny
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Biocatalysis
Biomarkers - metabolism
Chemistry
Domestic
Exact sciences and technology
Injuries
Injury analysis
Lactate dehydrogenase
Liver
Liver Diseases - metabolism
Logic
NAD - metabolism
Oxidation-Reduction
Probability
Spectrometric and optical methods
Swine
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Summary:A biocatalytic cascade for the analysis of the simultaneous increase in the concentration of two biomarkers characteristic of liver injury (alanine transaminase, ALT, and lactate dehydrogenase, LDH) was tested on real samples acquired from an animal model (domestic pigs, Sus scrofa domesticus) suffering from traumatic liver injury. A two-step reaction biocatalyzed in the presence of both enzyme-biomarkers resulted in the oxidation of NADH followed by optical absorbance measurements. A simple qualitative, YES/NO, test allowed for distinction between animals with and without the presence of liver injury with the probability of 92%. These data represent the first demonstration of applying binary logic systems for the analysis of real biomedical samples.
ISSN:0003-2654
1364-5528
DOI:10.1039/c2an00014h